ArticleInternational journal of molecular sciences2016
Isolation and Characterization of Dromedary Camel Coronavirus UAE-HKU23 from Dromedaries of the Middle East: Minimal Serological Cross-Reactivity between MERS Coronavirus and Dromedary Camel Coronavirus UAE-HKU23.
Article in International journal of molecular sciences, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
18 citing papers in PubMed, 26 citations in OpenAlex.
- Assessment and sero-diagnosis for coronaviruses with risk of human spillover.Emerging microbes & infections · 2023Article
- SARS-CoV-2 prevalence in domestic and wildlife animals: A genomic and docking based structural comprehensive review.Heliyon · 2023Review
- Serological Screening for Middle East Respiratory Syndrome Coronavirus and Hepatitis E Virus in Camels in Kazakhstan.Pathogens (Basel, Switzerland) · 2022Article
- A Sensitive and Specific Competitive Enzyme-Linked Immunosorbent Assay for Serodiagnosis of COVID-19 in Animals.Microorganisms · 2021Article
- Coronavirus Infections of Animals: Future Risks to Humans.Biology bulletin of the Russian Academy of Sciences · 2021Article
- Co-circulation of a Novel Dromedary Camel Parainfluenza Virus 3 and Middle East Respiratory Syndrome Coronavirus in a Dromedary Herd With Respiratory Tract Infections.Frontiers in microbiology · 2021Article
- Bovine Coronavirus and the Associated Diseases.Frontiers in veterinary science · 2021Review
- Analysis of knowledge bases and research hotspots of coronavirus from the perspective of mapping knowledge domain.Medicine · 2020Article
- Diversity of Dromedary Camel Coronavirus HKU23 in African Camels Revealed Multiple Recombination Events among Closely Related Betacoronaviruses of the Subgenus Embecovirus.Journal of virology · 2019Article
- Some One Health based control strategies for the Middle East respiratory syndrome coronavirus.One health (Amsterdam, Netherlands) · 2019Review
- Article
- Crystal structure of the S1 subunit N-terminal domain from DcCoV UAE-HKU23 spike protein.Virology · 2019Article
- Bovine-like coronaviruses in domestic and wild ruminants.Animal health research reviews · 2018Review
- Replicative virus shedding in the respiratory tract of patients with Middle East respiratory syndrome coronavirus infection.International journal of infectious diseases : IJID : official publication of the International Society for Infectious Diseases · 2018Article
- Rapid detection of MERS coronavirus-like viruses in bats: pote1ntial for tracking MERS coronavirus transmission and animal origin.Emerging microbes & infections · 2018Article
- Hosts and Sources of Endemic Human Coronaviruses.Advances in virus research · 2018Review
- Coronavirus infections in horses in Saudi Arabia and Oman.Transboundary and emerging diseases · 2017Article
- Middle East respiratory syndrome (MERS) coronavirus and dromedaries.Veterinary journal (London, England : 1997) · 2017Review
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
12 authors at 3 institutions in 3 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Recently, we reported the discovery of a dromedary camel coronavirus UAE-HKU23 (DcCoV UAE-HKU23) from dromedaries in the Middle East. In this study, DcCoV UAE-HKU23 was successfully isolated in two of the 14 dromedary fecal samples using HRT-18G cells, with cytopathic effects observed five days after inoculation. Northern blot analysis revealed at least seven distinct RNA species, corresponding to predicted subgenomic mRNAs and confirming the core sequence of transcription regulatory sequence motifs as 5'-UCUAAAC-3' as we predicted previously. Antibodies against DcCoV UAE-HKU23 were detected in 58 (98.3%) and 59 (100%) of the 59 dromedary sera by immunofluorescence and neutralization antibody tests, respectively. There was significant correlation between the antibody titers determined by immunofluorescence and neutralization assays (Pearson coefficient = 0.525, p < 0.0001). Immunization of mice using recombinant N proteins of DcCoV UAE-HKU23 and Middle East respiratory syndrome coronavirus (MERS-CoV), respectively, and heat-inactivated DcCoV UAE-HKU23 showed minimal cross-antigenicity between DcCoV UAE-HKU23 and MERS-CoV by Western blot and neutralization antibody assays. Codon usage and genetic distance analysis of RdRp, S and N genes showed that the 14 strains of DcCoV UAE-HKU23 formed a distinct cluster, separated from those of other closely related members of Betacoronavirus 1, including alpaca CoV, confirming that DcCoV UAE-HKU23 is a novel member of Betacoronavirus 1.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.