ArticleInternational journal of molecular sciences2016
A High Redox Potential Laccase from Pycnoporus sanguineus RP15: Potential Application for Dye Decolorization.
Article in International journal of molecular sciences, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
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16 citing papers in PubMed, 67 citations in OpenAlex.
- Exploration and heterologous expression of laccase genes and pesticide degradation ability of laccases from Cerrena unicolor GC.u01.World journal of microbiology & biotechnology · 2025Article
- Article
- Article
- Evaluating the efficacy of wood decay fungi and synthetic fungal consortia for simultaneous decolorization of multiple textile dyes.World journal of microbiology & biotechnology · 2023Article
- Production of a novel laccase from Ceratorhiza hydrophila and assessing its potential in natural dye fixation and cytotoxicity against tumor cells.Journal, genetic engineering & biotechnology · 2023Article
- Article
- Optimizing Covalent Immobilization of Glucose Oxidase and Laccase on PV15 Fluoropolymer-Based Bioelectrodes.Journal of functional biomaterials · 2022Article
- Review
- Biodecolorization and Ecotoxicity Abatement of Disperse Dye-Production Wastewater Treatment withInternational journal of environmental research and public health · 2022Article
- The production of laccases by white-rot fungi under solid-state fermentation conditions.World journal of microbiology & biotechnology · 2022Review
- A Brief History of Colour, the Environmental Impact of Synthetic Dyes and Removal by Using Laccases.Molecules (Basel, Switzerland) · 2021Review
- Heterologous Expression, Engineering and Characterization of a Novel Laccase ofJournal of fungi (Basel, Switzerland) · 2021Article
- Laccase isoform diversity on basal medium in Cyathus bulleri and role in decolorization/detoxification of textile dyes and effluent.World journal of microbiology & biotechnology · 2020Article
- Fungal Laccase Production from Lignocellulosic Agricultural Wastes by Solid-State Fermentation: A Review.Microorganisms · 2019Review
- Construction of a Robust Cofactor Self-Sufficient Bienzyme Biocatalytic System for Dye Decolorization and its Mathematical Modeling.International journal of molecular sciences · 2019Article
- Decolorization and Detoxification of Synthetic Dyes by Mexican Strains ofInternational journal of environmental research and public health · 2019Article
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Authors and funding
9 authors at 1 institution in 1 country.
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No grant is acknowledged in the PubMed record.
Abstract
Laccase production by Pycnoporus sanguineus RP15 grown in wheat bran and corncob under solid-state fermentation was optimized by response surface methodology using a Central Composite Rotational Design. A laccase (Lacps1) was purified and characterized and the potential of the pure Lacps1 and the crude culture extract for synthetic dye decolorization was evaluated. At optimal conditions (eight days, 26 °C, 18% (w/w) milled corncob, 0.8% (w/w) NH₄Cl and 50 mmol·L(-1) CuSO₄, initial moisture 4.1 mL·g(-1)), the laccase activity reached 138.6 ± 13.2 U·g(-1). Lacps1 was a monomeric glycoprotein (67 kDa, 24% carbohydrate). Optimum pH and temperature for the oxidation of 2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonate) (ABTS) were 4.4 and 74.4 °C, respectively. Lacps1 was stable at pH 3.0-8.0, and after two hours at 55-60 °C, presenting high redox potential (0.747 V vs. NHE). ABTS was oxidized with an apparent affinity constant of 147.0 ± 6.4 μmol·L(-1), maximum velocity of 413.4 ± 21.2 U·mg(-1) and catalytic efficiency of 3140.1 ± 149.6 L·mmol(-1)·s(-1). The maximum decolorization percentages of bromophenol blue (BPB), remazol brilliant blue R and reactive blue 4 (RB4), at 25 or 40 °C without redox mediators, reached 90%, 80% and 60%, respectively, using either pure Lacps1 or the crude extract. This is the first study of the decolorization of BPB and RB4 by a P. sanguineus laccase. The data suggested good potential for treatment of industrial dye-containing effluents.
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