ArticleASAIO journal (American Society for Artificial Internal Organs : 1992)
Achieving 12 Hour Normothermic Ex Situ Heart Perfusion: An Experience of 40 Porcine Hearts.
Article in ASAIO journal (American Society for Artificial Internal Organs : 1992). The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers.
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Who cites it
21 citing papers in PubMed, 51 citations in OpenAlex.
- Ex situ heart perfusion: a novel model for drug validation and translation.Frontiers in cardiovascular medicine · 2026Article
- Optimizing Temperature in Ex Situ Heart Perfusion: A Comparative Review of Traditional and Novel Methods in Heart Transplantation.Journal of cardiovascular development and disease · 2026Review
- Optimal Perfusion Pressure Enhances Donor Heart Preservation During Normothermic Ex Situ Perfusion in a Rat Transplantation Model.Medicina (Kaunas, Lithuania) · 2025Article
- Ex situ heart perfusion: A novel platform to test cardiovascular therapeutics in human hearts.JHLT open · 2025Article
- Donor Heart Preservation for Heart Transplantation: Single-Center Experience with Three Different Techniques.Journal of clinical medicine · 2025Article
- Identification of pharmacological inducers of a reversible hypometabolic state for whole organ preservation.eLife · 2024Article
- Successful Resuscitation of Porcine Hearts After 12 and 24 h of Static Cold Storage With Normothermic Ex Situ Perfusion.Transplantation direct · 2024Article
- Macrophage-derived extracellular vesicles alter cardiac recovery and metabolism in a rat heart model of donation after circulatory death.Journal of cellular and molecular medicine · 2024Article
- Novel portable hypothermic machine perfusion preservation device enhances cardiac viability of donated human hearts.Frontiers in cardiovascular medicine · 2024Article
- Extending heart preservation to 24 h with normothermic perfusion.Frontiers in cardiovascular medicine · 2024Article
- Electrophysiological Markers ofTransplant international : official journal of the European Society for Organ Transplantation · 2024Article
- Ex vivo normothermic perfusion of isolated segmental porcine bowel: a novel functional model of the small intestine.BJS open · 2021Article
- A Multi-Mode System for Myocardial Functional and Physiological Assessment during Ex Situ Heart Perfusion.The journal of extra-corporeal technology · 2020Article
- Hemodynamic adaptation of heart failure to percutaneous venoarterial extracorporeal circulatory supports.Physiological research · 2020Review
- Bioengineering approaches to organ preservation ex vivo.Experimental biology and medicine (Maywood, N.J.) · 2019Review
- Development of an Ex-Situ Limb Perfusion System for a Rodent Model.ASAIO journal (American Society for Artificial Internal Organs : 1992) · 2019Article
- Organ preservation: from the past to the future.Acta pharmacologica Sinica · 2018Review
- Machine perfusion of thoracic organs.Journal of thoracic disease · 2018Review
- Tachycardia-Induced Cardiomyopathy As a Chronic Heart Failure Model in Swine.Journal of visualized experiments : JoVE · 2018Article
- A New Multi-Mode Perfusion System for Ex Vivo Heart Perfusion Study.Journal of medical systems · 2017Article
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Authors and funding
12 authors at 3 institutions in 1 country.
Funding
Abstract
Although total body perfusion with extracorporeal life support (ECLS) can be maintained for weeks, individual organ perfusion beyond 12 hours has yet to be achieved clinically. Normothermic ex situ heart perfusion (ESHP) offers the potential for prolonged cardiac preservation. We developed an ESHP system to study the effect of perfusate variables on organ preservation, with the ultimate goal of extending organ perfusion for ≥24 hours. Forty porcine hearts were perfused for a target of 12 hours. Hearts that maintained electromechanical activity and had a <3× increase in vascular resistance were considered successful preservations. Perfusion variables, metabolic byproducts, and histopathology were monitored and sampled to identify factors associated with preservation failure. Twenty-two of 40 hearts were successfully preserved at 12 hours. Successful 12 hour experiments demonstrated lower potassium (4.3 ± 0.8 vs. 5.0 ± 1.2 mmol/L; p = 0.018) and lactate (3.5 ± 2.8 vs. 4.5 ± 2.9 mmol/L; p = 0.139) levels, and histopathology revealed less tissue damage (p = 0.003) and less weight gain (p = 0.072). Results of these early experiments suggest prolonged ESHP is feasible, and that elevated lactate and potassium levels are associated with organ failure. Further studies are necessary to identify the ideal perfusate for normothermic ESHP.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.