Evidence map›Paper›PMID 27163263›Full record

ArticleThe Analyst2016

Multi-scale silica structures for improved HIV-1 Capsid (p24) antigen detection.

Sophia Lin, Per Niklas Hedde, Vasan Venugopalan, Enrico Gratton, Michelle Khine

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in The Analyst, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

5 authors.

Sophia LinDepartment of Chemical Engineering and Materials Science, University of California, Irvine, Irvine, CA 92697, USA. mkhine@uci.edu.
Per Niklas HeddeDepartment of Biomedical Engineering, University of California, Irvine, Irvine, CA 92697, USA.
Vasan VenugopalanDepartment of Chemical Engineering and Materials Science, University of California, Irvine, Irvine, CA 92697, USA. mkhine@uci.edu and Department of Biomedical Engineering, University of California, Irvine, Irvine, CA 92697, USA.
Enrico GrattonDepartment of Biomedical Engineering, University of California, Irvine, Irvine, CA 92697, USA.
Michelle KhineDepartment of Chemical Engineering and Materials Science, University of California, Irvine, Irvine, CA 92697, USA. mkhine@uci.edu and Department of Biomedical Engineering, University of California, Irvine, Irvine, CA 92697, USA.

Funding

Uncover Strategies Used By Biological SystemsP50GM076516 · NIGMS · UNIVERSITY OF CALIFORNIA-IRVINE · PI NIE, QING · 2007 to 2016
$25.1M
TRD4: Phasor analysisP41GM103540 · NIGMS · UNIVERSITY OF CALIFORNIA-IRVINE · PI GRATTON, ENRICO · 2012 to 2020
$12.5M
Virtual Photonocs TechnologiesP41EB015890 · NIBIB · UNIVERSITY OF CALIFORNIA-IRVINE · PI DURKIN, ANTHONY · 2012 to 2017
$7.0M
Shrink Induced Manufacturing Platform for Low-Cost Diagnostics (SIMPL-CD)DP2OD007283 · OD · UNIVERSITY OF CALIFORNIA-IRVINE · PI KHINE, MICHELLE · 2010 to 2013
$2.4M
NIBIB NIH HHS P41 EB015890NIGMS NIH HHS P41 GM103540NIGMS NIH HHS P50 GM076516NIH HHS DP2 OD007283
6 · The paper itself

Abstract

Silica (SiO2) micro- and nanostructures fabricated with pre-stressed thermoplastic shrink wrap film have been shown to yield far-field fluorescence signal enhancements over their planar or wrinkled counterparts. The SiO2 structures have previously been used for improved detection of fluorescently labelled proteins and DNA. In this work, we probe the mechanism responsible for the dramatic increases in fluorescence signal intensity. Optical characterization studies attribute the fluorescence signal enhancements to increased surface density and light scattering from the rough SiO2 structures. Using this information, we come up with a theoretical approximation for the enhancement factor based off the scattering effects alone. We show that increased deposition thickness of SiO2 yields improved fluorescence signal enhancements, with an optimal SiO2 thin layer achieved at 20 nm. Finally, we show that the SiO2 substrates serve as a suitable platform for disease diagnostics, and show improved limits of detection (LOD) for the human immunodeficiency virus type 1 (HIV-1) p24 antigen.

Indexed as

HIV-1NanostructuresSilicon DioxideCapsidHIV Core Protein p24Limit of DetectionHIV Core Protein p24p24 protein, Human Immunodeficiency Virus Type 1Silicon Dioxide

Identifiers

PMID27163263
PMCPMC4924925

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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.