ArticleInternational journal of ophthalmology2016
Expression of indoleamine 2,3-dioxygenase in a murine model of Aspergillus fumigatus keratitis.
Article in International journal of ophthalmology, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 9 papers.
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Who cites it
9 citing papers in PubMed, 11 citations in OpenAlex.
- The Role of Biofilms in Contact Lens Associated Fungal Keratitis.Antibiotics (Basel, Switzerland) · 2023Article
- Indoleamine 2,3-dioxygenase adjusts neutrophils recruitment and chemotaxis inInternational journal of ophthalmology · 2022Article
- Update on the management of fungal keratitis.International ophthalmology · 2021Review
- Immunologic mechanism of fungal keratitis.International journal of ophthalmology · 2021Review
- Review
- Indoleamine 2,3-Dioxygenase Regulates Macrophage Recruitment, Polarization and Phagocytosis in Aspergillus Fumigatus Keratitis.Investigative ophthalmology & visual science · 2020Article
- Expression and role of aryl hydrocarbon receptor inInternational journal of ophthalmology · 2020Article
- Rodent Models of Invasive Aspergillosis due toFrontiers in microbiology · 2017Review
- A Multifaceted Role of Tryptophan Metabolism and Indoleamine 2,3-Dioxygenase Activity inFrontiers in immunology · 2017Review
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Authors and funding
10 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
aimTo observe the presence and expression of indoleamine 2,3-dioxygenase (IDO) during the corneal immunity to Aspergillus fumigatus (A. fumigatus) in the murine models.
methodsThe murine model of fungal keratitis was established by smearing with colonies of A. fumigatus after scraping central epithelium of cornea and covering with contact lenses in C57BL/6 mice. The mice were randomly divided into control group, sham group and A. fumigatus keratitis group. The cornea was monitored daily using a slit lamp and recorded disease score after infection. Corneal lesion was detected by immunofluorescence staining. IDO mRNA and protein were also detected by quantitative reverse transcription-polymerase chain reaction (qRT-PCR) and Western blot.
resultsThe disease score and slit lamp photography indicated that disease severity was consistent with corneal inflammation in the murine models, and the disease scores in A. fumigatus keratitis group were obviously higher than those in the sham group. By immunofluorescence staining, IDO was mainly localized in corneal epithelium and stroma in the murine corneal tissues with A. fumigatus keratitis. Compared with the sham group, IDO mRNA expression was significantly enhanced in corneal epithelium infected by A. fumigatus. Furthermore, IDO protein expression detected by Western blot was in accord with transcript levels of IDO mRNA measured by qRT-PCR. IDO protein expression was enhanced after A. fumigatus infection compared with the sham group.
conclusionIDO is detected in corneal epithelium and stroma locally, which indicates IDO takes part in the pathogenesis of A. fumigatus keratitis and plays a key role in immune regulation at the early stage.
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