Evidence map›Paper›PMID 27162495›Full record

ArticleBreeding science2016

Utilization of flow cytometry for festulolium breeding (Lolium multiflorum (2x) × Festuca arundinacea (6x)).

Yukio Akiyama, Yasufumi Ueyama, Seiya Hamada, Akito Kubota, Daisuke Kato, Hitomi Yamada-Akiyama, Yoshinori Takahara, Masahiro Fujimori

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Breeding science, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed.

  1. Plants (Basel, Switzerland) · 2023
    Article
  2. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

8 authors.

Yukio AkiyamaNational Research and Development Agency, National Agriculture and Food Research Organization (NARO), Tohoku Agricultural Research Center, 4 Akahira, Shimokuriyagawa, Morioka, Iwate 020-0198, Japan; The United Graduate School of Agricultural Sciences, Iwate University, 3-18-8 Ueda, Morioka, Iwate 020-8550, Japan.
Yasufumi UeyamaNational Research and Development Agency, National Agriculture and Food Research Organization (NARO), Institute of Livestock and Grassland Science (NILGS) , 768 Senbonmatsu, Nasushiobara, Tochigi 329-2793 , Japan.
Seiya HamadaDepartment of BioEngineering, Nagaoka University of Technology , 1603-1 Kamitomioka-cho, Nagaoka, Niigata 940-2188 , Japan.
Akito KubotaNational Research and Development Agency, National Agriculture and Food Research Organization (NARO), Tohoku Agricultural Research Center , 4 Akahira, Shimokuriyagawa, Morioka, Iwate 020-0198 , Japan.
Daisuke KatoNational Research and Development Agency, National Agriculture and Food Research Organization (NARO), Tohoku Agricultural Research Center , 4 Akahira, Shimokuriyagawa, Morioka, Iwate 020-0198 , Japan.
Hitomi Yamada-AkiyamaFaculty of Agriculture, Iwate University , 3-18-8 Ueda, Morioka, Iwate 020-8550 , Japan.
Yoshinori TakaharaDepartment of BioEngineering, Nagaoka University of Technology , 1603-1 Kamitomioka-cho, Nagaoka, Niigata 940-2188 , Japan.
Masahiro FujimoriNational Research and Development Agency, National Agriculture and Food Research Organization (NARO), Tohoku Agricultural Research Center, 4 Akahira, Shimokuriyagawa, Morioka, Iwate 020-0198, Japan; The United Graduate School of Agricultural Sciences, Iwate University, 3-18-8 Ueda, Morioka, Iwate 020-8550, Japan.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Festulolium is a hybrid between Festuca and Lolium species that has valuable agronomic traits from both grass species. The purpose of our breeding program is to produce hexaploid festulolium that introduces tolerance to summer depression into Italian ryegrass (Lolium multiflorum) by crossing it with tall fescue (Festuca arundinacea). However, we found the DNA ploidy of hexaploids was not stable and was reduced in successive generations. We aimed to find out how to obtain stable high-ploidy festulolium. F1 hybrids of L. multiflorum and F. arundinacea were produced. The F3 generation was produced from putative hexaploid F2 individuals by open pollination. The F4 to F6 generations were obtained by polycrossing. The DNA ploidy levels of F2 to F6 individuals were estimated by flow cytometry. Cytological characteristics of the F5 and F6 individuals were investigated by FISH and GISH. The DNA ploidy level of hexaploid festulolium was reduced and stabilized at almost the same level as a tetraploid. Seed fertility was inversely correlated with an increase in ploidy level. GISH revealed no preferential Lolium transmission. FISH with a telomere probe revealed that counting the exact number of chromosomes in festulolium was difficult. DNA ploidy level was strongly correlated with the number of chromosomes.

Indexed as

DNA ploidyFestuca arundinaceafestuloliumflow cytometry (FCM)fluorescence in situ hybridization (FISH)genomic in situ hybridization (GISH)Lolium multiflorum

Identifiers

PMID27162495
PMCPMC4785001

What OpenQuestion holds

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.