ArticleScientific reports2016
Metabolic engineering of a synergistic pathway for n-butanol production in Saccharomyces cerevisiae.
Article in Scientific reports, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 26 papers.
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Who cites it
26 citing papers in PubMed, 84 citations in OpenAlex.
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- Model-Guided Rational Construction of Escherichia coli Synthetic Consortia for Enhanced 2-Methylbutyric Acid Production.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2025Article
- Harnessing organelle engineering to facilitate biofuels and biochemicals production in yeast.Journal of microbiology (Seoul, Korea) · 2025Review
- Production of (R)-citramalate by engineeredMetabolic engineering communications · 2024Article
- CRISPRi-induced transcriptional regulation of IAH1 gene and its influence on volatile compounds profile in Kluyveromyces marxianus DU3.World journal of microbiology & biotechnology · 2024Article
- Increased production of isobutanol from xylose through metabolic engineering of Saccharomyces cerevisiae overexpressing transcription factor Znf1 and exogenous genes.FEMS yeast research · 2024Article
- Engineering yeast mitochondrial metabolism for 3-hydroxypropionate production.Biotechnology for biofuels and bioproducts · 2023Article
- PlasmidMaker is a versatile, automated, and high throughput end-to-end platform for plasmid construction.Nature communications · 2022Article
- Enhancing biofuels production by engineering the actin cytoskeleton in Saccharomyces cerevisiae.Nature communications · 2022Article
- Experimental investigation on n-butanol/methyl oleate dual fuel RCCI combustion in a single cylinder engine at high-load condition.Scientific reports · 2021Article
- Metabolic engineering of Deinococcus radiodurans for pinene production from glycerol.Microbial cell factories · 2021Article
- Enhanced sucrose fermentation by introduction of heterologous sucrose transporter and invertase intoRoyal Society open science · 2021Article
- Butanol Tolerance ofGenes · 2021Article
- n-Butanol production by Saccharomyces cerevisiae from protein-rich agro-industrial by-products.Brazilian journal of microbiology : [publication of the Brazilian Society for Microbiology] · 2020Article
- Genetic engineering of non-native hosts for 1-butanol production and its challenges: a review.Microbial cell factories · 2020Review
- Butanol production by Saccharomyces cerevisiae: perspectives, strategies and challenges.World journal of microbiology & biotechnology · 2020Review
- Synthetic Biology and Metabolic Engineering EmployingFrontiers in bioengineering and biotechnology · 2020Review
- CRISPR/Cas9 Systems for the Development ofFrontiers in bioengineering and biotechnology · 2020Review
- Metabolic engineering ofBiotechnology for biofuels · 2019Article
Corrections and comments
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Authors and funding
6 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
n-Butanol has several favourable properties as an advanced fuel or a platform chemical. Bio-based production of n-butanol is becoming increasingly important for sustainable chemical industry. Synthesis of n-butanol can be achieved via more than one metabolic pathway. Here we report the metabolic engineering of Saccharomyces cerevisiae to produce n-butanol through a synergistic pathway: the endogenous threonine pathway and the introduced citramalate pathway. Firstly, we characterized and optimized the endogenous threonine pathway; then, a citramalate synthase (CimA) mediated pathway was introduced to construct the synergistic pathway; next, the synergistic pathway was optimized by additional overexpression of relevant genes identified previously; meanwhile, the n-butanol production was also improved by overexpression of keto-acid decarboxylases (KDC) and alcohol dehydrogenase (ADH). After combining these strategies with co-expression of LEU1 (two copies), LEU4, LEU2 (two copies), LEU5, CimA, NFS1, ADH7 and ARO10(*), we achieved an n-butanol production of 835 mg/L in the final engineered strain, which is almost 7-fold increase compared to the initial strain. Furthermore, the production showed a 3-fold of the highest titer ever reported in yeast. Therefore, the engineered yeast strain represents a promising alternative platform for n-butanol production.
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