ArticleParasites & vectors2016
Vertical transmission of Anaplasma platys and Leishmania infantum in dogs during the first half of gestation.
Article in Parasites & vectors, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
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Who cites it
16 citing papers in PubMed, 29 citations in OpenAlex.
- Development of hexaplex PCR assay for the detection and characterization common tick-borne pathogens in dogs, and analysis of risk factors.Veterinary and animal science · 2026Article
- Sire risk factors for vertical transmission of Leishmania infantum by the dam.PLoS neglected tropical diseases · 2026Article
- Endoparasitic Diseases in Breeding Kennels: A Frequent and Complex Problem Requiring a Holistic Approach.Animals : an open access journal from MDPI · 2024Review
- Strategies for the Diagnosis of Granulocytic Anaplasmosis in Two Naturally Infected Dogs.Animals : an open access journal from MDPI · 2023Article
- The estimated distribution of autochthonous leishmaniasis by Leishmania infantum in Europe in 2005-2020.PLoS neglected tropical diseases · 2023Review
- Dead or Alive? A Review of Perinatal Factors That Determine Canine Neonatal Viability.Animals : an open access journal from MDPI · 2022Review
- Epidemiology, Diagnosis, and Control of Canine Infectious Cyclic Thrombocytopenia and Granulocytic Anaplasmosis: Emerging Diseases of Veterinary and Public Health Significance.Veterinary sciences · 2021Review
- Prevalence of Vector-Borne Pathogens in Reproductive and Non-Reproductive Tissue Samples from Free-Roaming Domestic Cats in the South Atlantic USA.Pathogens (Basel, Switzerland) · 2021Article
- Review
- Molecular Survey of Vector-Borne Pathogens of Dogs and Cats in Two Regions of Saudi Arabia.Pathogens (Basel, Switzerland) · 2020Article
- Article
- A review on the eco-epidemiology and clinical management of human granulocytic anaplasmosis and its agent in Europe.Parasites & vectors · 2019Review
- Evidence for vertical transmission of Mycoplasma haemocanis, but not Ehrlichia ewingii, in a dog.Journal of veterinary internal medicine · 2019Article
- Seroprevalence and risk factors associated with Ehrlichia canis, Anaplasma spp., Borrelia burgdorferi sensu lato, and D. immitis in hunting dogs from southern Italy.Parasitology research · 2017Article
- Potential Challenges of Controlling Leishmaniasis in Sri Lanka at a Disease Outbreak.BioMed research international · 2017Review
- Nephrin gene expression in chronic kidney disease of dogs with Leishmania (Leishmania) infantum chagasi.The Brazilian journal of infectious diseases : an official publication of the Brazilian Society of Infectious DiseasesArticle
Corrections and comments
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Authors and funding
8 authors at 5 institutions in 4 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundLeishmania infantum is a canine zoonotic vector-borne protozoan pathogen transmitted by phlebotomine sand flies, whereas Anaplasma platys is a bacterium most likely transmitted by ticks. While vertical transmission of L. infantum from pregnant bitches to their offspring has been documented, thus far no studies have explored the possibility of vertical transmission of A. platys in dogs. This study investigated the occurrence of vertical transmission of L. infantum and A. platys in sheltered dogs during the first half of gestation, in an area of southern Italy characterised by a high incidence of infection by both pathogens.
methodsThe study population included 20 bitches (n = 10 pregnant, at 25-35 days of pregnancy; n = 10 non-pregnant), all subjected to ovariohysterectomy, which were examined for the presence of L. infantum and A. platys via cytological screening of bone marrow and whole blood samples. Infection by L. infantum and A. platys was also tested by immunofluorescence antibody test (IFAT) and quantitative real-time PCR (qPCR) targeting both pathogens. Selected tissue samples (n = 210) collected during surgical procedures from bitches and foetuses (n = 20) were assessed for the presence of L. infantum and A. platys by qPCR targeting a fragment of the kinetoplast minicircle DNA (kDNA) and the 16S rRNA gene, respectively.
resultsLeishmania infantum DNA was not amplified from either uteri or ovaries from pregnant bitches or foetal tissue samples, whereas a subset of ovarian (n = 2) and uterine (n = 4) tissue samples from non-pregnant bitches were infected, with parasite loads of up to 3.09 × 10 and 7.51 parasite/PCR reaction, respectively. Conversely, uterine (n = 10) and ovarian (n = 8) tissues from both pregnant and non-pregnant bitches, together with a subset (n = 5) of foetal tissue samples were qPCR positive for A. platys. Leishmania infantum and A. platys nucleic acids were amplified from two uteri from non-pregnant bitches, with parasite loads of up to 2.32 × 10(-3) and 2.05 parasite/per PCR reaction, respectively.
conclusionsResults from this study suggest that, in contrast to L. infantum, A. platys can be transmitted from pregnant dogs to their offspring during the first half of gestation. This hypothesis remains to be verified, for instance via direct observation of parasites in postpartum foetal tissues.
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