ArticleThe Journal of membrane biology1989
Integral and peripheral protein composition of the apical and basolateral membrane domains in MDCK cells.
Article in The Journal of membrane biology, 1989. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 83 papers.
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Who cites it
83 citing papers in PubMed, 231 citations in OpenAlex.
- Sonic hedgehog is basolaterally sorted from the TGN and transcytosed to the apical domain involving Dispatched-1 at Rab11-ARE.Frontiers in cell and developmental biology · 2022Article
- Inside-out regulation of E-cadherin conformation and adhesion.Proceedings of the National Academy of Sciences of the United States of America · 2021Article
- Localization of ZIP14 and ZIP8 in HIBCPP Cells.Brain sciences · 2020Article
- The Role of Tricellulin in Epithelial Jamming and Unjamming via Segmentation of Tricellular Junctions.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2020Article
- Glucocorticoids and myosin5b loss of function induce heightened PKA signaling in addition to membrane traffic defects.Molecular biology of the cell · 2019Article
- Clathrin and clathrin adaptor AP-1 control apical trafficking of megalin in the biosynthetic and recycling routes.Molecular biology of the cell · 2019Article
- Quantitative proteomics of MDCK cells identify unrecognized roles of clathrin adaptor AP-1 in polarized distribution of surface proteins.Proceedings of the National Academy of Sciences of the United States of America · 2019Article
- Article
- Basolateral sorting of chloride channel 2 is mediated by interactions between a dileucine motif and the clathrin adaptor AP-1.Molecular biology of the cell · 2015Article
- Plasma membrane protein polarity and trafficking in RPE cells: past, present and future.Experimental eye research · 2014Review
- Basolateral targeting and microtubule-dependent transcytosis of the aquaporin-2 water channel.American journal of physiology. Cell physiology · 2013Article
- Hepatocyte polarity.Comprehensive Physiology · 2013Review
- Proinflammatory cytokine secretion is suppressed by TMEM16A or CFTR channel activity in human cystic fibrosis bronchial epithelia.Molecular biology of the cell · 2012Article
- The clathrin adaptor AP-1A mediates basolateral polarity.Developmental cell · 2012Article
- Helicobacter pylori perturbs iron trafficking in the epithelium to grow on the cell surface.PLoS pathogens · 2011Article
- Basolateral sorting signals regulating tissue-specific polarity of heteromeric monocarboxylate transporters in epithelia.Traffic (Copenhagen, Denmark) · 2011Article
- Overcoming key technological challenges in using mass spectrometry for mapping cell surfaces in tissues.Molecular & cellular proteomics : MCP · 2011Review
- Targeting and imaging signature caveolar molecules in lungs.Proceedings of the American Thoracic Society · 2009Article
- A CD317/tetherin-RICH2 complex plays a critical role in the organization of the subapical actin cytoskeleton in polarized epithelial cells.The Journal of cell biology · 2009Article
- Surface expression of epithelial Na channel protein in rat kidney.The Journal of general physiology · 2008Article
23 more citing papers are in PubMed but not listed here.
Corrections and comments
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Authors and funding
5 authors at 2 institutions in 1 country.
Funding
Abstract
Selective biotinylation of the apical or basolateral domains of confluent MDCK monolayers grown on polycarbonate filters with a water soluble biotin analog, sulfo-NHS-biotin, was employed to reveal strikingly distinct patterns of endogenous "peripheral" and "integral" membrane proteins. "Peripheral" proteins were found to be approximately fivefold more abundant with this procedure than "integral" membrane proteins, both on the apical and on the basolateral surface. The distinct apical and basal patterns were shown to depend upon the integrity of the monolayer; when the tight junctions were disrupted by preincubation in calcium-depleted medium, the patterns appeared practically indistinguishable. Two-dimensional gel electrophoresis demonstrated that only a very small percentage of the biotinylated proteins were found in similar amounts on both apical and basolateral domains. These results indicate that the sorting mechanisms that segregate apical and basolateral epithelial proteins are very strict. The simple procedure described here has clear advantages over other methods available to label apical and basal epithelial surface domains, namely, higher accessibility of the biotin probe to the basolateral membrane, possibility of purifying biotinylated proteins via immobilized streptavidin and minimal exposure of the researcher to isotopes. It should be very useful in characterizing the apical and basolateral protein compositions of other epithelial cells and in studies on the development of epithelial cell polarity.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.