ArticleScientific reports2016
Hot spots of DNA double-strand breaks in human rDNA units are produced in vivo.
Article in Scientific reports, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
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Who cites it
16 citing papers in PubMed.
- The Nucleolus in Human Disease: Ribosome Biogenesis, Nucleolar Surveillance, and Therapeutic Opportunities.Biomolecules · 2026Review
- Mitotic BLM functions are required to maintain genomic stability.Nucleic acids research · 2026Article
- Nucleolar expansion: A biomolecular condensate mortality timer.Geromedicine · 2026Article
- Hypermethylation at 45S rDNA promoter in cancers.PloS one · 2025Article
- CBP and RAD21 Proteins Bind at the Termini of Forum Domains in Human Chromosomes.Doklady. Biochemistry and biophysics · 2023Article
- RAD51-mediated R-loop formation acts to repair transcription-associated DNA breaks driving antigenic variation inProceedings of the National Academy of Sciences of the United States of America · 2023Article
- Under the magnifying glass: The ups and downs of rDNA copy number.Seminars in cell & developmental biology · 2023Review
- Genes Possessing the Most Frequent DNA DSBs Are Highly Associated with Development and Cancers, and Essentially Overlap with the rDNA-Contacting Genes.International journal of molecular sciences · 2022Article
- Article
- The Role of rDNA Clusters in Global Epigenetic Gene Regulation.Frontiers in genetics · 2021Review
- Hedgehog signaling enables repair of ribosomal DNA double-strand breaks.Nucleic acids research · 2020Article
- Ribosomal biogenesis as an emerging target of neurodevelopmental pathologies.Journal of neurochemistry · 2019Review
- The epigenetic regulator SIRT7 guards against mammalian cellular senescence induced by ribosomal DNA instability.The Journal of biological chemistry · 2018Article
- A role for Tau protein in maintaining ribosomal DNA stability and cytidine deaminase-deficient cell survival.Nature communications · 2017Article
- Single nucleotide-level mapping of DNA double-strand breaks in human HEK293T cells.Genomics data · 2017Article
- Fine resolution mapping of double-strand break sites for human ribosomal DNA units.Genomics data · 2016Article
Corrections and comments
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Authors and funding
9 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Endogenous hot spots of DNA double-strand breaks (DSBs) are tightly linked with transcription patterns and cancer genomics(1,2). There are nine hot spots of DSBs located in human rDNA units(3-6). Here we describe that the profiles of these hot spots coincide with the profiles of γ-H2AX or H2AX, strongly suggesting a high level of in vivo breakage inside rDNA genes. The data were confirmed by microscopic observation of the largest γ-H2AX foci inside nucleoli in interphase chromosomes. In metaphase chromosomes, we observed that only some portion of rDNA clusters possess γ-H2AX foci and that all γ-H2AX foci co-localize with UBF-1 binding sites, which strongly suggests that only active rDNA units possess the hot spots of DSBs. Both γ-H2AX and UBF-1 are epigenetically inherited and thus indicate the rDNA units that were active in the previous cell cycle. These results have implications for diverse fields, including epigenetics and cancer genomics.
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