ArticleParasites & vectors2016
Molecular detection of Leishmania infantum, filariae and Wolbachia spp. in dogs from southern Portugal.
Article in Parasites & vectors, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers, 1 of them a synthesis that pooled it.
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Who cites it
20 citing papers in PubMed, 1 synthesis or guideline pooled it, 36 citations in OpenAlex.
- Canine Leishmaniasis in Europe over the Last Decade: A Review of Geographic Trends and Epidemiological Data.Pathogens (Basel, Switzerland) · 2025Pooled it
- Six-month field efficacy and safety of the combined treatment of dogs with Frontline Tri-Act® and NexGard Spectra®.Parasites & vectors · 2018Trial
- Knowledge and practices of dog and cat owners in Mainland Portugal regarding fleas, flea-borne pathogens, and their management.Parasites & vectors · 2025Article
- Molecular detection of Leishmania donovani, Dirofilaria sp. "hongkongensis," and Wolbachia spp. in the dog population from tribal settlements of Western Ghats, Kerala, India.Parasitology research · 2025Article
- Distribution of and Relationships between Epidemiological and Clinicopathological Parameters in Canine Leishmaniosis: A Retrospective Study of 15 Years (2009-2023).Pathogens (Basel, Switzerland) · 2024Article
- Global Distribution of Canine Visceral Leishmaniasis and the Role of the Dog in the Epidemiology of the Disease.Pathogens (Basel, Switzerland) · 2024Review
- Interaction between Wolbachia pipientis and Leishmania infantum in heartworm infected dogs.Parasites & vectors · 2023Article
- Seroprevalence and Risk Factors Associated withMicroorganisms · 2022Article
- Detection of Wolbachia and Leishmania DNA in sand flies (Diptera: Psychodidae, Phlebotominae) from a focus of cutaneous leishmaniasis in Tabasco, Mexico.Parasitology research · 2022Article
- Epidemiologic, Clinical and Immunological Consequences of Co-Infections during Canine Leishmaniosis.Animals : an open access journal from MDPI · 2021Review
- Simultaneous Exposure toPathogens (Basel, Switzerland) · 2021Article
- Haematological and biochemical abnormalities in hunting dogs infected with Acanthocheilonema reconditum, associated risk factors, and a European overview.Parasitology research · 2021Article
- Article
- New Molecular Data on Filaria and itsPathogens (Basel, Switzerland) · 2020Article
- Development of a multiplex qPCR-based approach for the diagnosis of Dirofilaria immitis, D. repens and Acanthocheilonema reconditum.Parasites & vectors · 2020Article
- Detection of Canine Vector-Borne Filariasis and TheirMicroorganisms · 2020Article
- Vector-borne nematode diseases in pets and humans in the Mediterranean Basin: An update.Veterinary world · 2019Review
- Prevalence of Anaplasmataceae and Filariidae species in unowned and military dogs in New Caledonia.Veterinary medicine and science · 2018Article
- Infection by Mycoplasma spp., feline immunodeficiency virus and feline leukemia virus in cats from an area endemic for visceral leishmaniasis.Parasites & vectors · 2018Article
- Evaluation of four molecular methods to detect Leishmania infection in dogs.Parasites & vectors · 2017Article
Corrections and comments
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Authors and funding
9 authors at 5 institutions in 3 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundLeishmaniosis caused by the protozoan Leishmania infantum and dirofilariosis caused by the nematodes Dirofilaria immitis or Dirofilaria repens are vector-borne zoonoses widely present in the Mediterranean basin. In addition, some studies reported that the endosymbiont Wolbachia spp. play a role in the biology and pathogenesis of filarial parasites. The aim of this work was to evaluate the frequency of mono- and co-infections by L. infantum, filariae and Wolbachia spp. and their association with clinical signs in dogs from the south of Portugal. Leishmanial, filarial and Wolbachia spp. DNA were evaluated by specific real-time polymerase chain reaction (qPCR) assays in blood samples from 230 dogs.
findingsOne hundred and thirty-nine (60.4 %) dogs were qPCR-positive for L. infantum and 26 (11.3 %) for filariae (24 for D. immitis only, one D. immitis and for Acanthocheilonema dracunculoides and another one for Acanthocheilonema reconditum only). Wolbachia spp. DNA was amplified from 16 (64.0 %) out of the 25 D. immitis-positive dogs. Nineteen (8.3 %) dogs were co-infected with L. infantum and D. immitis, including the one (0.4 %) A. drancunculoides-positive animal. In dogs without clinical signs consistent with leishmaniosis and/or dirofilariosis, L. infantum prevalence was 69 %, whereas in those dogs with at least one clinical manifestation compatible with any of the two parasitoses prevalence was 42.7 %. Leishmania prevalence was significantly higher in apparently healthy mongrels (77.2 %) and pets (76.9 %) than in defined-breed dogs (including crosses; 58.8 %) and in dogs with an aptitude other than pet (i.e. farm, guard, hunting, shepherd or stray), respectively, whereas in those dogs with at least one clinical sign, the detection of L. infantum DNA was higher in males (53.3 %) and in those dogs not receiving insect repellents (52.8 %).
conclusionsThe molecular detection of canine vector-borne disease (CVBD) agents, some of which are zoonotic, reinforces the need to implement efficient prophylactic measures, such as insect repellents and macrocyclic lactones (including compliance to administration), in the geographical areas where these agents are distributed, with the view to prevent infection and disease among mammalian hosts including humans.
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