ArticleBiopreservation and biobanking2016
DNA Source Selection for Downstream Applications Based on DNA Quality Indicators Analysis.
Article in Biopreservation and biobanking, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 93 papers.
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Who cites it
93 citing papers in PubMed.
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- A high-yield, SPE-free DNA extraction method using a pyridinium-based ionic liquid compatible with downstream PCR analysis.Analytical and bioanalytical chemistry · 2026Article
- Genome-wide DAPC-based genetic structure, linkage disequilibrium, and effective population size in indigenous buffaloes of Northeastern and Central India.Mammalian genome : official journal of the International Mammalian Genome Society · 2026Article
- DNA purity over yield: optimizing extraction and PCR in degraded Asian elephant (Elephas maximus) fecal samples.Genes & genomics · 2026Article
- Dried blood spot-based monitoring of immune and epigenetic biomarkers in occupational exposure studies.Journal of exposure science & environmental epidemiology · 2026Article
- Metabolic Flexibility and Exercise Performance in Adults with Gilbert's Syndrome-Associated Hyperbilirubinemia.Sports medicine - open · 2026Article
- Assessment of High Throughput Sequencing Quality of Host DNA Enriched From Faeces: A Case From Captive Tiger.Molecular ecology resources · 2026Article
- DNA Recovery from Forensically Relevant Blow Fly Larvae (Insecta, Diptera, Calliphoridae) Kept in Different Preservative Solutions.Neotropical entomology · 2026Article
- Development of a Gold Nanoparticle-Based Amplification-Free Nanobiosensor for Rapid DNA Detection Supported by Machine Learning.Biosensors · 2026Article
- Vancomycin-resistant enterococci in healthcare settings: clonal analysis, resistance profiles, and biofilm formation of strains isolated from hospitalized patients.Folia microbiologica · 2026Article
- Molecular Characterization ofJournal of parasitology research · 2026Article
- Circulating extracellular vesicle DNA (EV-DNA) and cell-free DNA in ovarian cancer: pathological relevance and diagnostic applications.Extracellular vesicles and circulating nucleic acids · 2026Review
- Naturally acquired malaria is associated with shorter leukocyte relative telomere length and increased hTERT expression in an endemic nigerian population.Frontiers in cellular and infection microbiology · 2026Article
- Metal accumulation in paired colon cancer and adjacent tissues and its relationship with genotoxic and epigenetic biomarkers.Frontiers in public health · 2026Article
- Effects of thermal exposure on bone surface characteristics and DNA recovery.Forensic science international. Synergy · 2025Article
- ApoE4 Homozygosity Is Associated With Increased Microglia Activation in Fatal COVID-19.Neuropathology : official journal of the Japanese Society of Neuropathology · 2025Article
- Benchmarking Cost-Effective DNA Extraction Kits for Diverse Metagenomic Samples.International journal of molecular sciences · 2025Article
- Oligotrophic bacteria and pathotrophic fungi moderate multitrophic interactions in semi-arid and arid environments.Environmental microbiome · 2025Article
33 more citing papers are in PubMed but not listed here.
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
6 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
High-quality human DNA samples and associated information of individuals are necessary for biomedical research. Biobanks act as a support infrastructure for the scientific community by providing a large number of high-quality biological samples for specific downstream applications. For this purpose, biobank methods for sample preparation must ensure the usefulness and long-term functionality of the products obtained. Quality indicators are the tool to measure these parameters, the purity and integrity determination being those specifically used for DNA. This study analyzes the quality indicators in DNA samples derived from 118 frozen human tissues in optimal cutting temperature (OCT) reactive, 68 formalin-fixed paraffin-embedded (FFPE) tissues, 119 frozen blood samples, and 26 saliva samples. The results obtained for DNA quality are discussed in association with the usefulness for downstream applications and availability of the DNA source in the target study. In brief, if any material is valid, blood is the most approachable option of prospective collection of samples providing high-quality DNA. However, if diseased tissue is a requisite or samples are available, the recommended source of DNA would be frozen tissue. These conclusions will determine the best source of DNA, according to the planned downstream application. Furthermore our results support the conclusion that a complete procedure of DNA quantification and qualification is necessary to guarantee the appropriate management of the samples, avoiding low confidence results, high costs, and a waste of samples.
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