Evidence map›Paper›PMID 27158554›Full record

ArticleJournal of analytical & bioanalytical techniques2015

A Novel [

Feng Jin, Salil Kumar Bhowmik, Vasanta Putluri, Franklin Gu, Jie Gohlke, Friedrich Carl Von Rundstedt, Subhamoy Dasgupta, Rashmi Krishnapuram, Bert W O'Malley, Arun Sreekumar and 1 more

Open access · hybridAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Journal of analytical & bioanalytical techniques, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
0.1field-weighted citation impact, top 44% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed, 2 citations in OpenAlex.

  1. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

11 authors at 1 institution in 1 country.

Feng JinAdvanced Technology Core, Baylor College of Medicine, One Baylor Plaza Houston, TX, USA; Alkek Center for Molecular Discovery, Baylor College of Medicine, One Baylor Plaza Houston, TX, USA.
Salil Kumar BhowmikDepartment of Molecular and Cell Biology, Baylor College of Medicine, One Baylor Plaza Houston, TX, USA; Alkek Center for Molecular Discovery, Baylor College of Medicine, One Baylor Plaza Houston, TX, USA.
Vasanta PutluriAdvanced Technology Core, Baylor College of Medicine, One Baylor Plaza Houston, TX, USA; Alkek Center for Molecular Discovery, Baylor College of Medicine, One Baylor Plaza Houston, TX, USA.
Franklin GuDepartment of Biochemistry, Baylor College of Medicine, One Baylor Plaza Houston, TX, USA.
Jie GohlkeDepartment of Molecular and Cell Biology, Baylor College of Medicine, One Baylor Plaza Houston, TX, USA; Alkek Center for Molecular Discovery, Baylor College of Medicine, One Baylor Plaza Houston, TX, USA.
Friedrich Carl Von RundstedtScott Department of Urology, Baylor College of Medicine, Houston, TX, USA; Helios Klinikum, Witten-Herdecke University, Wuppertal, Germany.
Subhamoy DasguptaDepartment of Molecular and Cell Biology, Baylor College of Medicine, One Baylor Plaza Houston, TX, USA.
Rashmi KrishnapuramDepartment of Molecular and Cell Biology, Baylor College of Medicine, One Baylor Plaza Houston, TX, USA.
Bert W O'MalleyDepartment of Molecular and Cell Biology, Baylor College of Medicine, One Baylor Plaza Houston, TX, USA; Alkek Center for Molecular Discovery, Baylor College of Medicine, One Baylor Plaza Houston, TX, USA.
Arun SreekumarAdvanced Technology Core, Baylor College of Medicine, One Baylor Plaza Houston, TX, USA; Department of Molecular and Cell Biology, Baylor College of Medicine, One Baylor Plaza Houston, TX, USA; Department of Biochemistry, Baylor College of Medicine, One Baylor Plaza Houston, TX, USA; Alkek Center for Molecular Discovery, Baylor College of Medicine, One Baylor Plaza Houston, TX, USA.
Nagireddy PutluriAdvanced Technology Core, Baylor College of Medicine, One Baylor Plaza Houston, TX, USA; Department of Molecular and Cell Biology, Baylor College of Medicine, One Baylor Plaza Houston, TX, USA; Alkek Center for Molecular Discovery, Baylor College of Medicine, One Baylor Plaza Houston, TX, USA.
Baylor College of Medicine · US

Funding

Tumor BiologyP30CA125123 · NCI · BAYLOR COLLEGE OF MEDICINE · PI DAVID R ROWLEY · 2007 to 2026
$73.9M
REPRODUCTIVE HORMONES: BIOLOGICAL AND MOLECULAR ACTIONSR01HD008188 · NICHD · BAYLOR COLLEGE OF MEDICINE · PI DAVID M LONARD, JOHN P LYDON · 1985 to 2026
$16.3M
METABOLOMIC PROFILING AND BIOLOGIC BASIS OF RACIAL DISPARITY IN PROSTATE CANCERU01CA167234 · NCI · BAYLOR COLLEGE OF MEDICINE · PI PALAPATTU, GANESH S, SREEKUMAR, ARUN · 2012 to 2016
$1.9M
Delineating racially distinct metabolic pathways in triple negative breast cancerU01CA179674 · NCI · BAYLOR COLLEGE OF MEDICINE · PI SREEKUMAR, ARUN · 2014 to 2018
$1.7M
Integrative Metabolomics of Prostate Cancer ProgressionR01CA133458 · NCI · UNIVERSITY OF MICHIGAN AT ANN ARBOR · PI SREEKUMAR, ARUN · 2008 to 2012
$1.6M
BCM Oncology Scholars Training ProgramT32CA174647 · NCI · BAYLOR COLLEGE OF MEDICINE · PI LIN, WEEI-CHIN · 2014 to 2018
$1.2M
High Kynurenine in Agressive Triple Negative African American Breast CancerR21CA185516 · NCI · BAYLOR COLLEGE OF MEDICINE · PI SREEKUMAR, ARUN · 2014 to 2015
$374k
NCI NIH HHS P30 CA125123NCI NIH HHS R01 CA133458NCI NIH HHS R21 CA185516NCI NIH HHS T32 CA174647NCI NIH HHS U01 CA167234NCI NIH HHS U01 CA179674NICHD NIH HHS R01 HD008188
6 · The paper itself

Abstract

The growth of cancer cells relies more on increased proliferation and autonomy compared to non-malignant cells. The rate of de novo nucleotide biosynthesis correlates with cell proliferation rates. In part, glutamine is needed to sustain high rates of cellular proliferation as a key nitrogen donor in purine and pyrimidine nucleotide biosynthesis. In addition, glutamine serves as an essential substrate for key enzymes involved in the de novo synthesis of purine and pyrimidine nucleotides. Here, we developed a novel liquid chromatography (LC-MS) to quantify glutamine-derived [15N] nitrogen flux into nucleosides and nucleobases (purines and pyrimidines). For this, DNA from 5637 bladder cancer cell line cultured in 15N labelled glutamine and then enzymatically hydrolyzed by sequential digestion. Subsequently, DNA hydrolysates were separated by LC-MS and Selected Reaction Monitoring (SRM) was employed to identify the nucleobases and nucleosides. Thus, high sensitivity and reproducibility of the method make it a valuable tool to identify the nitrogen flux primarily derived from glutamine and can be further adaptable for high throughput analysis of large set of DNA in a clinical setting.

Indexed as

Bladder cancer cell linesGlutamine nitrogen fluxNucleosides and nucleobases biosynthesisTandem mass spectrometry

Identifiers

PMID27158554
PMCPMC4857768
OpenAlexW2468716009

What OpenQuestion holds

Textmetadata
LicenceTDM
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.