ArticleAmerican journal of translational research2016
The GroEL protein of Porphyromonas gingivalis regulates atherogenic phenomena in endothelial cells mediated by upregulating toll-like receptor 4 expression.
Article in American journal of translational research, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.
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Who cites it
20 citing papers in PubMed, 35 citations in OpenAlex.
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- Unveiling the Molecular Crosstalk Between Periodontal and Cardiovascular Diseases: A Systematic Review.Dentistry journal · 2025Review
- Alzheimer's Disease andLife (Basel, Switzerland) · 2025Review
- Human Coronary Artery Endothelial Cell Response toMicroorganisms · 2024Article
- Integrative analysis of gene and protein expression in atherosclerosis-related pathways modulated by periodontal pathogens. Systematic review.The Japanese dental science review · 2023Review
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- The pathogenic mechanism of oral bacteria and treatment with inhibitors.Clinical and experimental dental research · 2022Review
- Molecular hydrogen suppresses Porphyromonas gingivalis lipopolysaccharide-induced increases in interleukin-1 alpha and interleukin-6 secretion in human gingival cells.Molecular and cellular biochemistry · 2022Article
- Periodontopathic Microbiota and Atherosclerosis: Roles of TLR-Mediated Inflammation Response.Oxidative medicine and cellular longevity · 2022Review
- Immune Mechanisms of Plaque Instability.Frontiers in cardiovascular medicine · 2021Review
- Observational
- [Effect ofBeijing da xue xue bao. Yi xue ban = Journal of Peking University. Health sciences · 2020Article
- Infection and atherosclerosis: TLR-dependent pathways.Cellular and molecular life sciences : CMLS · 2020Review
- Prediction of novel target genes and pathways involved in bevacizumab-resistant colorectal cancer.PloS one · 2018Article
- Porphyromonas gingivalis lipopolysaccharides act exclusively through TLR4 with a resilience between mouse and human.Scientific reports · 2017Article
- Tanshinone IIA Attenuates Atherosclerosis in Apolipoprotein E Knockout Mice Infected with Porphyromonas gingivalis.Inflammation · 2017Article
- Eotaxin-2 increased toll-like receptor 4 expression in endothelial cellsAmerican journal of translational research · 2016Article
- Endothelial Response to Glucocorticoids in Inflammatory Diseases.Frontiers in immunology · 2016Review
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Authors and funding
10 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Porphyromonas gingivalis (P. gingivalis) is a bacterial species that causes periodontitis. GroEL from P. gingivalis may possess biological activity and may be involved in the destruction of periodontal tissues. However, it is unclear whether P. gingivalis GroEL enhances the appearance of atherogenic phenomena in endothelial cells and vessels. Here, we constructed recombinant GroEL from P. gingivalis to investigate its effects in human coronary artery endothelial cells (HCAECs) in vitro and on aortas of high-cholesterol (HC)-fed B57BL/6 and B57BL/6-Tlr4(lps-del) mice in vivo. The results showed that GroEL impaired tube-formation capacity under non-cytotoxic conditions in HCAECs. GroEL increased THP-1 cell/HCAEC adhesion by increasing the expression of intracellular adhesion molecule (ICAM)-1 and vascular adhesion molecule (VCAM)-1 in endothelial cells. Additionally, GroEL increased DiI-oxidized low density lipoprotein (oxLDL) uptake, which may be mediated by elevated lectin-like oxLDL receptor (LOX)-1 but not scavenger receptor expressed by endothelial cells (SREC) and scavenger receptor class B1 (SR-B1) expression. Furthermore, GroEL interacts with toll-like receptor 4 (TLR4) and plays a causal role in atherogenesis in HCAECs. Human antigen R (HuR), an RNA-binding protein with a high affinity for the 3' untranslated region (3'UTR) of TLR4 mRNA, contributes to the up-regulation of TLR4 induced by GroEL in HCAECs. In a GroEL animal administration study, GroEL elevated ICAM-1, VCAM-1, LOX-1 and TLR4 expression in the aortas of HC diet-fed wild C57BL/6 but not C57BL/6-Tlr4(lps-del) mice. Taken together, our findings suggest that P. gingivalis GroEL may contribute to cardiovascular disorders by affecting TLR4 expression.
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27158334PMC4846891W2392493340What OpenQuestion holds
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