ArticleJournal of ginseng research2016
Pectinase-treated Panax ginseng ameliorates hydrogen peroxide-induced oxidative stress in GC-2 sperm cells and modulates testicular gene expression in aged rats.
Article in Journal of ginseng research, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers, 1 of them a synthesis that pooled it.
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Who cites it
17 citing papers in PubMed, 1 synthesis or guideline pooled it, 36 citations in OpenAlex.
- Pooled it
- 2,8-Dihydroxyquinoline ameliorates oligoasthenozoospermia by inhibiting MAPK14-mediated spermatogonial ferroptosis.Molecular biology reports · 2026Article
- Guilu erxian glue improves sperm quality in oligasthenospermia mice by promoting TDRP methylation and regulating the cAMP/PKA pathway.Scientific reports · 2025Article
- Cordycepin mitigates spermatogenic and redox related expression in HPharmaceutical biology · 2022Article
- Ginseng alleviates folliculogenesis disorders via induction of cell proliferation and downregulation of apoptotic markers in nicotine-treated mice.Journal of ovarian research · 2022Article
- Effects ofExperimental and therapeutic medicine · 2020Article
- Ginseng for Improving Semen Quality Parameters: A Systematic Review.The world journal of men's health · 2020Article
- Cordycepin, an Active Constituent of Nutrient Powerhouse and Potential Medicinal MushroomNutrients · 2019Article
- Shengjing Capsule Improves Spermatogenesis through Upregulating IntegrinEvidence-based complementary and alternative medicine : eCAM · 2019Article
- Protective effects of Korean Red Ginseng against sub-acute immobilization stress-induced testicular damage in experimental rats.Journal of ginseng research · 2019Article
- The effects of phenolic glycosides from Betula platyphylla var. japonica on adipocyte differentiation and mature adipocyte metabolism.Journal of enzyme inhibition and medicinal chemistry · 2018Article
- Tirucallane Triterpenoids from the Stems and Stem Bark ofMolecules (Basel, Switzerland) · 2018Article
- Chemical Characterization of a Renoprotective Metabolite from Termite-Associated Streptomyces sp. RB1 against Cisplatin-Induced Cytotoxicity.International journal of molecular sciences · 2018Article
- Article
- Ginseng: An Nonnegligible Natural Remedy for Healthy Aging.Aging and disease · 2017Review
- Effects of ginseng on two main sex steroid hormone receptors: estrogen and androgen receptors.Journal of ginseng research · 2017Review
- Prokineticin 2 overexpression induces spermatocyte apoptosis in varicocele in rats.Asian journal of andrologyArticle
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Authors and funding
7 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundTo investigate the effect of pectinase-treated Panax ginseng (GINST) in cellular and male subfertility animal models.
methodsHydrogen peroxide (H2O2)-induced mouse spermatocyte GC-2spd cells were used as an in vitro model. Cell viability was measured using MTT assay. For the in vivo study, GINST (200 mg/kg) mixed with a regular pellet diet was administered orally for 4 mo, and the changes in the mRNA and protein expression level of antioxidative and spermatogenic genes in young and aged control rats were compared using real-time reverse transcription polymerase chain reaction and western blotting.
resultsGINST treatment (50 μg/mL, 100 μg/mL, and 200 μg/mL) significantly (p < 0.05) inhibited the H2O2-induced (200 μM) cytotoxicity in GC-2spd cells. Furthermore, GINST (50 μg/mL and 100 μg/mL) significantly (p < 0.05) ameliorated the H2O2-induced decrease in the expression level of antioxidant enzymes (peroxiredoxin 3 and 4, glutathione S-transferase m5, and glutathione peroxidase 4), spermatogenesis-related protein such as inhibin-α, and specific sex hormone receptors (androgen receptor, luteinizing hormone receptor, and follicle-stimulating hormone receptor) in GC-2spd cells. Similarly, the altered expression level of the above mentioned genes and of spermatogenesis-related nectin-2 and cAMP response element-binding protein in aged rat testes was ameliorated with GINST (200 mg/kg) treatment. Taken together, GINST attenuated H2O2-induced oxidative stress in GC-2 cells and modulated the expression of antioxidant-related genes and of spermatogenic-related proteins and sex hormone receptors in aged rats.
conclusionGINST may be a potential natural agent for the protection against or treatment of oxidative stress-induced male subfertility and aging-induced male subfertility.
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