Evidence map›Paper›PMID 27158200›Full record

ArticleWorld journal of gastroenterology2016

Growth hormone abolishes the negative effects of everolimus on intestinal wound healing.

Markus Alexander Küper, Sebastian Trütschel, Jürgen Weinreich, Alfred Königsrainer, Stefan Beckert

Open access · hybridAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in World journal of gastroenterology, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 3 papers.

0numbers the graph read from it
0cells of the map it votes in
3citing papers in PubMed
0.5field-weighted citation impact, top 31% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

3 citing papers in PubMed, 6 citations in OpenAlex.

  1. A WR3-NHMaterials today. Bio · 2026
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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

5 authors at 2 institutions in 1 country.

Markus Alexander KüperMarkus Alexander Küper, Sebastian Trütschel, Jürgen Weinreich, Alfred Königsrainer, Stefan Beckert, Department of General, Visceral and Transplant Surgery, University of Tübingen, 72076 Tübingen, Germany.
Sebastian TrütschelMarkus Alexander Küper, Sebastian Trütschel, Jürgen Weinreich, Alfred Königsrainer, Stefan Beckert, Department of General, Visceral and Transplant Surgery, University of Tübingen, 72076 Tübingen, Germany.
Jürgen WeinreichMarkus Alexander Küper, Sebastian Trütschel, Jürgen Weinreich, Alfred Königsrainer, Stefan Beckert, Department of General, Visceral and Transplant Surgery, University of Tübingen, 72076 Tübingen, Germany.
Alfred KönigsrainerMarkus Alexander Küper, Sebastian Trütschel, Jürgen Weinreich, Alfred Königsrainer, Stefan Beckert, Department of General, Visceral and Transplant Surgery, University of Tübingen, 72076 Tübingen, Germany.
Stefan BeckertMarkus Alexander Küper, Sebastian Trütschel, Jürgen Weinreich, Alfred Königsrainer, Stefan Beckert, Department of General, Visceral and Transplant Surgery, University of Tübingen, 72076 Tübingen, Germany.
University of Tübingen · DEBG Klinik Tübingen · DE

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

aimTo investigate whether the simultaneous treatment with human growth hormone (hGH) abolishes the negative effects of everolimus on anastomotic healing.

methodsForty-eight male Sprague-Dawley-rats were randomized to three groups of 16 animals each (I: vehicle; II: everolimus 3 mg/kg po; III: everolimus 3 mg/kg po + hGH 2.5 mg/kg sc). Animals were pre-treated with hGH and/or everolimus daily for seven days. Then a standard anastomosis was created in the descending colon and treatment was continued for another seven days. The anastomosis was resected in toto and the bursting pressure was assessed as a mechanical parameter of intestinal healing. Moreover, biochemical (Hydroxyproline, PCNA, MPO, MMP-2 and MMP-9) and histological (cell density, angiogenesis, amount of granulation tissue) parameters of intestinal healing were assessed.

resultsAnastomotic bursting pressure was significantly reduced by everolimus and a simultaneous treatment with hGH resulted in considerably higher values (I: 134 ± 19 mmHg, II: 85 ± 25 mmHg, III: 114 ± 25 mmHg; P < 0.05, I vs II; P = 0.09, I vs III and II vs III) Hydroxyproline concentration was significantly increased by hGH compared to everolimus alone (I: 14.9 ± 2.5 μg/mg, II: 8.9 ± 3.6 μg/mg, III: 11.9 ± 2.8 μg/mg; P < 0.05, I vs II/III and II vs III). The number of MPO-positive cells was reduced significantly by hGH compared to everolimus alone (I: 10 ± 1 n/mm², II: 15 ± 3 n/mm², III: 9 ± 2 n/mm²; P < 0.05, I vs II and II vs III), while the number of PCNA-positive cells were increased by hGH (I: 28 ± 3 /mm², II: 12 ± 3 /mm², III: 26 ± 12 /mm²; P < 0.05, I vs II and II vs III). Corresponding to these biochemical findings, HE-histology revealed significantly increased amount of granulation tissue in hGH-treated animals.

conclusionInhibition of intestinal wound healing by everolimus is partially neutralized by simultaeous treatment with hGH. Both inflammation as well as collagen deposition is influenced by hGH.

Indexed as

Anastomosis, SurgicalAnimalsEverolimusHuman Growth HormoneHydroxyprolineIntestinesMalePeroxidaseProliferating Cell Nuclear AntigenRatsRats, Sprague-DawleyTOR Serine-Threonine KinasesWound HealingEverolimusHuman Growth HormoneHydroxyprolinePeroxidaseProliferating Cell Nuclear AntigenTOR Serine-Threonine KinasesAnastomotic healingEverolimusGrowth hormoneHuman growth hormoneImmunosuppressionmTOR-inhibitorWound healing

Identifiers

PMID27158200
PMCPMC4853689
OpenAlexW2346414817

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.