ArticleNature communications2016
Multimodal two-photon imaging using a second harmonic generation-specific dye.
Article in Nature communications, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 23 papers.
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Who cites it
23 citing papers in PubMed.
- Ordered Polar Topological Domains Enabling Giant Second-Harmonic Generation in Ferroelectric Nematic Liquid Crystals.Advanced materials (Deerfield Beach, Fla.) · 2026Article
- Second harmonic imaging of membrane potentials: Comparing water and chromophores as probes.Biophysical journal · 2026Article
- Intravital Imaging of Immune Responses in the Cancer Microenvironment.Cancer medicine · 2025Review
- Interleaflet Translocation of Second-Harmonic-Generation-Active Dye Molecules in Phospholipid Bilayers with Transmembrane Pores.Langmuir : the ACS journal of surfaces and colloids · 2025Article
- Endogenous and exogenous wireless multimodal light-emitting chemical devices.Chemical science · 2023Article
- Stimulated Raman scattering microscopy reveals a unique and steady nature of brain water dynamics.Cell reports methods · 2023Article
- Organic Kainate Single Crystals for Second-Harmonic and Broadband THz Generation.ACS applied materials & interfaces · 2023Article
- Monitoring membranes: The exploration of biological bilayers with second harmonic generation.Chemical physics reviews · 2022Review
- Tools to measure membrane potential of neurons.Biomedical journal · 2022Review
- Combined Second Harmonic Generation and Fluorescence Analyses of the Structures and Dynamics of Molecules on Lipids Using Dual-Probes: A Review.Molecules (Basel, Switzerland) · 2022Review
- Monolignol export by diffusion down a polymerization-induced concentration gradient.The Plant cell · 2022Article
- Two-Photon Absorption: An Open Door to the NIR-II Biological Window?Frontiers in chemistry · 2022Review
- Two-dimensional electronic-vibrational sum frequency spectroscopy for interactions of electronic and nuclear motions at interfaces.Proceedings of the National Academy of Sciences of the United States of America · 2021Article
- Applications of second harmonic generation (SHG)/sum-frequency generation (SFG) imaging for biophysical characterization of the plasma membrane.Biophysical reviews · 2020Review
- Multimodal optical contrast agents as new tools for monitoring and tuning nanoemulsion internalisation into cancer cells. From live cell imaging toNanoscale advances · 2020Article
- Second Harmonic Generation Spectroscopy of Membrane Probe Dynamics in Gram-Positive Bacteria.Biophysical journal · 2019Article
- Optical second harmonic generation microscopy: application to the sensitive detection of cell membrane damage.Biophysical reviews · 2019Review
- Harmonic Generation Microscopy 2.0: New Tricks Empowering Intravital Imaging for Neuroscience.Frontiers in molecular biosciences · 2019Review
- Article
- Porphyrin Dyes for Nonlinear Optical Imaging of Live Cells.iScience · 2018Article
Corrections and comments
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Authors and funding
6 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Second harmonic generation (SHG) imaging can be used to visualize unique biological phenomena, but currently available dyes limit its application owing to the strong fluorescent signals that they generate together with SHG. Here we report the first non-fluorescent and membrane potential-sensitive SHG-active organic dye Ap3. Ap3 is photostable and generates SH signals at the plasma membrane with virtually no fluorescent signals, in sharp contrast to the previously used fluorescent dye FM4-64. When tested in neurons, Ap3-SHG shows linear membrane potential sensitivity and fast responses to action potentials, and also shows significantly reduced photodamage compared with FM4-64. The SHG-specific nature of Ap3 allows simultaneous and completely independent imaging of SHG signals and fluorescent signals from various reporter molecules, including markers of cellular organelles and intracellular calcium. Therefore, this SHG-specific dye enables true multimodal two-photon imaging in biological samples.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.