ArticleJournal of assisted reproduction and genetics2016
Growth and differentiation factor 9 promotes oocyte growth at the primary but not the early secondary stage in three-dimensional follicle culture.
Article in Journal of assisted reproduction and genetics, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 10 papers.
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Who cites it
10 citing papers in PubMed, 28 citations in OpenAlex.
- Biological Functions of Growth Differentiation Factor 9 in Early Ovarian Development of Japanese Eel (Anguilla japonica).Marine biotechnology (New York, N.Y.) · 2025Article
- The Roles of GDF-9, BMP-15, BMP-4 and EMMPRIN in Folliculogenesis and In Vitro Fertilization.Journal of clinical medicine · 2024Review
- The Presence of TGFβ3 in Human Ovarian Intrafollicular Fluid and Its Involvement in Thromboxane Generation in Follicular Granulosa Cells through a Canonical TGFβRI, Smad2/3 Signaling Pathway and COX-2 Induction.International journal of molecular sciences · 2024Article
- Improvement of Mouse Preantral Follicle Survival and Development following Co-Culture with Ovarian Parenchyma Cell Suspension.International journal of fertility & sterility · 2024Article
- The relationship between G1 (c.260 G>A) and G4 (c.721 G>A) polymorphisms in the GDF9 gene and the litter size of sheep: A meta-analysis study.Journal of advanced veterinary and animal research · 2023Article
- Advances in Oocyte Maturation In Vivo and In Vitro in Mammals.International journal of molecular sciences · 2023Review
- In vitro production of viable eggs from isolated mouse primary follicles by successive culture.The Journal of reproduction and development · 2022Article
- Involvement of Phosphorylated Akt and FOXO3a in the Effects of Growth and Differentiation Factor-9 (GDF-9) on Inhibition of Follicular Apoptosis and Induction of Granulosa Cell Proliferation After In Vitro Culture of Sheep Ovarian Tissue.Reproductive sciences (Thousand Oaks, Calif.) · 2021Article
- The effect of the human cumulus cells-conditioned medium on in vitro maturation of mouse oocyte: An experimental study.International journal of reproductive biomedicine · 2020Article
- Assessing recrudescence of photoregressed Siberian hamster ovaries using in vitro whole ovary culture.Molecular reproduction and development · 2018Article
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Authors and funding
5 authors at 1 institution in 1 country.
Funding
Abstract
purposeFactors that differentially regulate oocyte and granulosa cell growth within the early preantral follicle and how these factors differ at each stage of follicle growth remain poorly understood. The aim of this study was to isolate and evaluate the effect of recombinant growth and differentiation factor 9 (GDF9) on oocyte and granulosa cell growth at the primary and early secondary stages of preantral follicle growth during in vitro culture.
methodsPrimary stage follicles (diameters of 50-89 μm) and early secondary stage follicles (diameters of 90-120 μm) were isolated from immature mice, and individual, intact follicles were cultured in vitro in the presence and absence of recombinant GDF9. The effects of GDF9 on follicle growth were determined by the assessment of changes in the follicle volume during culture. The growth of the granulosa cell and oocyte compartments of the follicles was evaluated separately at each stage.
resultsGDF9 significantly increased the growth of isolated follicles at both the primary and early secondary follicle stages. Independent evaluation of the granulosa cell and oocyte compartments revealed that, while GDF9 promoted granulosa cell growth at both stages of folliculogenesis, oocyte growth was stage specific. GDF9 promoted growth of the oocyte at the primary, but not the early secondary, follicle stage.
conclusionsThese findings demonstrate a stage-specific role for GDF9 in the regulation of oocyte and granulosa cell growth at the primary and early secondary stages of preantral follicle development.
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