Evidence map›Paper›PMID 27154760›Full record

ArticleJournal of industrial microbiology & biotechnology2016

Analysis of respiratory activity and carbon usage of a mutant of Azotobacter vinelandii impaired in poly-β-hydroxybutyrate synthesis.

Lucero Jiménez, Tania Castillo, Celia Flores, Daniel Segura, Enrique Galindo, Carlos Peña

Abstract read
PubMed Publisher
In one paragraph

Article in Journal of industrial microbiology & biotechnology, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 5 papers.

0numbers the graph read from it
0cells of the map it votes in
5citing papers in PubMed
0.7field-weighted citation impact, top 28% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

5 citing papers in PubMed, 12 citations in OpenAlex.

  1. Advances in alginate biosynthesis: regulation and production inFrontiers in bioengineering and biotechnology · 2025
    Review
  2. Article
  3. Genetic Regulation of Alginate Production inFrontiers in microbiology · 2022
    Review
  4. Article
  5. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

6 authors at 2 institutions in 1 country.

Lucero JiménezDepartamento de Ingeniería Celular y Biocatálisis, Instituto de Biotecnología, Universidad Nacional Autónoma de México, Apdo. Post. 510-3, 62250, Cuernavaca, Morelos, Mexico.
Tania CastilloCentro de Investigaciones en Biotecnología, Universidad Autónoma del Estado de Morelos, Av. Universidad 1001, Col. Chamilpa C.P, 62209, Cuernavaca, Morelos, Mexico.
Celia FloresDepartamento de Ingeniería Celular y Biocatálisis, Instituto de Biotecnología, Universidad Nacional Autónoma de México, Apdo. Post. 510-3, 62250, Cuernavaca, Morelos, Mexico.
Daniel SeguraDepartamento de Microbiología Molecular, Instituto de Biotecnología, Universidad Nacional Autónoma de México, Apdo. Post. 510-3, 62250, Cuernavaca, Morelos, Mexico.
Enrique GalindoDepartamento de Ingeniería Celular y Biocatálisis, Instituto de Biotecnología, Universidad Nacional Autónoma de México, Apdo. Post. 510-3, 62250, Cuernavaca, Morelos, Mexico.
Carlos PeñaDepartamento de Ingeniería Celular y Biocatálisis, Instituto de Biotecnología, Universidad Nacional Autónoma de México, Apdo. Post. 510-3, 62250, Cuernavaca, Morelos, Mexico. carlosf@ibt.unam.mx.
Universidad Nacional Autónoma de México · MXUniversidad Autónoma del Estado de Morelos · MX

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

In this study, the respiratory activity and carbon usage of the mutant strain of A. vinelandii AT6, impaired in poly-β-hydroxybutyrate (PHB) production, and their relationship with the synthesis of alginate were evaluated. The alginate yield and the specific oxygen uptake rate were higher (2.5-fold and 62 %, respectively) for the AT6 strain, compared to the control strain (ATCC 9046), both in shake flasks cultures and in bioreactor, under fixed dissolved oxygen tension (1 %). In contrast, the degree of acetylation was similar in both strains. These results, together with the analysis of carbon usage (% C-mol), suggest that in the case of the AT6 strain, the flux of acetyl-CoA (precursor molecule for PHB biosynthesis and alginate acetylation) was diverted to the respiratory chain passing through the tricarboxylic acids cycle, and an important % C-mol was directed through alginate biosynthesis, up to 25.9 % and to a lesser extent, to biomass production (19.7 %).

Indexed as

Acetyl Coenzyme AAlginatesAzotobacter vinelandiiCarbonGlucuronic AcidHexuronic AcidsHydroxybutyratesMutationOxygenPolyestersPolyhydroxybutyratesAcetyl Coenzyme AAlginatesCarbonGlucuronic AcidHexuronic AcidsHydroxybutyratesOxygenpoly-beta-hydroxybutyratePolyestersPolyhydroxybutyratesAlginate productionAzotobacter vinelandiiCarbon usagePoly(β-hydroxybutyrate)Respiratory activity

Identifiers

PMID27154760
OpenAlexW2346143710

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.