ArticleCurrent microbiology2016
Enhancing Indigo Production by Over-Expression of the Styrene Monooxygenase in Pseudomonas putida.
Article in Current microbiology, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 11 papers.
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Who cites it
11 citing papers in PubMed, 39 citations in OpenAlex.
- Tryptophan-Based Hyperproduction of Bioindigo by Combinatorial Overexpression of Two Different Tryptophan Transporters.Journal of microbiology and biotechnology · 2024Article
- Indigo production goes green: a review on opportunities and challenges of fermentative production.World journal of microbiology & biotechnology · 2024Review
- A Combinational Optimization Method for Efficient Production of Indigo by the RecombinantFoods (Basel, Switzerland) · 2023Article
- Production of Indigo by RecombinantFoods (Basel, Switzerland) · 2022Article
- Investigation of indole biodegradation by Cupriavidus sp. strain IDO with emphases on downstream biotransformation and indigo production.Environmental science and pollution research international · 2022Article
- Efficient Bioproduction of Indigo and Indirubin by Optimizing a Novel Terpenoid Cyclase XiaI inACS omega · 2021Article
- An overview of microbial indigo-forming enzymes.Applied microbiology and biotechnology · 2020Review
- Structure-Based Redesign of a Self-Sufficient Flavin-Containing Monooxygenase towards Indigo Production.International journal of molecular sciences · 2019Article
- Two-Component FAD-Dependent Monooxygenases: Current Knowledge and Biotechnological Opportunities.Biology · 2018Review
- On the Enigma of Glutathione-Dependent Styrene Degradation in Gordonia rubripertincta CWB2.Applied and environmental microbiology · 2018Article
- Biodegradation and Biotransformation of Indole: Advances and Perspectives.Frontiers in microbiology · 2018Review
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Authors and funding
6 authors at 2 institutions in 1 country.
Funding
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Abstract
As an important traditional blue dye, indigo has been used in food and textile industry for centuries, which can be produced via the styrene oxygenation pathway in Pseudomonas putida. Hence, the styrene monooxygenase gene styAB and oxide isomerase gene styC are over-expressed in P. putida to investigate their roles in indigo biosynthesis. RT-qPCR analysis indicated that transcriptions of styA and styB were increased by 2500- and 750-folds in the styAB over-expressed strain B4-01, compared with the wild-type strain B4, consequently significantly enhancing the indole monooxygenase activity. Transcription of styC was also increased by 100-folds in the styC over-expressed strain B4-02. Besides, styAB over-expression slightly up-regulated the transcription of styC in B4-01, while styC over-expression hardly exerted an effect on the transcriptional levels of styA and styB and indole monooxygenase activity in B4-02. Furthermore, shaking flask experiments showed that indigo production in B4-01 reached 52.13 mg L(-1) after 24 h, which was sevenfold higher than that in B4. But no obvious increase in indigo yield was observed in B4-02. Over-expression of styAB significantly enhanced the indigo production, revealing that the monooxygenase STYAB rather than oxide isomerase STYC probably acted as the key rate-limiting enzyme in the indigo biosynthesis pathway in P. putida. This work provided a new strategy for enhancing indigo production in Pseudomonas.
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