ArticlePloS one2016
Stochastic Assembly of Bacteria in Microwell Arrays Reveals the Importance of Confinement in Community Development.
Article in PloS one, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. An erratum has been issued. Cited by 24 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
24 citing papers in PubMed.
- PEG-Based Living Hydrogels Engineered for Tunable Production of Bioactive Lipopeptides.ACS applied polymer materials · 2026Article
- Surface-Imprinted Polymer Coupled with Diffraction Gratings for Low-Cost, Label-Free and DifferentialBiosensors · 2026Article
- A digital plating platform for robust and versatile microbial detection and analysis.Scientific reports · 2025Article
- Habitat fragmentation enhances microbial collective defence.Journal of the Royal Society, Interface · 2025Article
- Photo-addressable microwell devices for rapid functional screening and isolation of pathogen inhibitors from bacterial strain libraries.Biomicrofluidics · 2024Article
- Microbiome engineering for bioremediation of emerging pollutants.Bioprocess and biosystems engineering · 2023Review
- Emerging platforms for high-throughput enzymatic bioassays.Trends in biotechnology · 2023Review
- Microfluidic devices for studying bacterial taxis, drug testing and biofilm formation.Microbial biotechnology · 2022Review
- Low-cost gel-filled microwell array device for screening marine microbial consortium.Frontiers in microbiology · 2022Article
- The effect of droplet size on syntrophic dynamics in droplet-enabled microbial co-cultivation.PloS one · 2022Article
- Video frame prediction of microbial growth with a recurrent neural network.Frontiers in microbiology · 2022Article
- A Review of Capillary Pressure Control Valves in Microfluidics.Biosensors · 2021Review
- Biopolymer Patterning-Directed Secretion in Mucoid and Nonmucoid Strains ofACS infectious diseases · 2021Article
- Micro-Technologies for Assessing Microbial Dynamics in Controlled Environments.Frontiers in microbiology · 2021Review
- Simultaneous Discovery of Positive and Negative Interactions Among Rhizosphere Bacteria Using Microwell Recovery Arrays.Frontiers in microbiology · 2020Article
- Experimental and computational approaches to unravel microbial community assembly.Computational and structural biotechnology journal · 2020Review
- Microbial Interaction Network Inference in Microfluidic Droplets.Cell systems · 2019Article
- A Multiwell Microfluidic Device for Analyzing and Screening Nonhormonal Contraceptive Agents.Small (Weinheim an der Bergstrasse, Germany) · 2019Article
- Fabricating Reactive Surfaces with Brush-like and Crosslinked Films of Azlactone-Functionalized Block Co-Polymers.Journal of visualized experiments : JoVE · 2018Article
- A Microfluidics and Agent-Based Modeling Framework for Investigating Spatial Organization in Bacterial Colonies: The Case ofFrontiers in microbiology · 2018Article
Corrections and comments
- Erratum issued
Authors and funding
9 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The structure and function of microbial communities is deeply influenced by the physical and chemical architecture of the local microenvironment and the abundance of its community members. The complexity of this natural parameter space has made characterization of the key drivers of community development difficult. In order to facilitate these characterizations, we have developed a microwell platform designed to screen microbial growth and interactions across a wide variety of physical and initial conditions. Assembly of microbial communities into microwells was achieved using a novel biofabrication method that exploits well feature sizes for control of innoculum levels. Wells with incrementally smaller size features created populations with increasingly larger variations in inoculum levels. This allowed for reproducible growth measurement in large (20 μm diameter) wells, and screening for favorable growth conditions in small (5, 10 μm diameter) wells. We demonstrate the utility of this approach for screening and discovery using 5 μm wells to assemble P. aeruginosa colonies across a broad distribution of innoculum levels, and identify those conditions that promote the highest probability of survivial and growth under spatial confinement. Multi-member community assembly was also characterized to demonstrate the broad potential of this platform for studying the role of member abundance on microbial competition, mutualism and community succession.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.