ArticleBiomaterials science2016
Enhanced non-vitreous cryopreservation of immortalized and primary cells by ice-growth inhibiting polymers.
Article in Biomaterials science, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
21 citing papers in PubMed, 49 citations in OpenAlex.
- Assay-ready Cryopreserved Cell Monolayers Enabled by Macromolecular Cryoprotectants.Biomacromolecules · 2022Article
- Proline pre-conditioning of cell monolayers increases post-thaw recovery and viability by distinct mechanisms to other osmolytes.RSC medicinal chemistry · 2021Article
- Ice Inhibition for Cryopreservation: Materials, Strategies, and Challenges.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2021Review
- Ice recrystallisation inhibiting polymers prevent irreversible protein aggregation during solvent-free cryopreservation as additives and as covalent polymer-protein conjugates.European polymer journal · 2020Article
- Post-Thaw Culture and Measurement of Total Cell Recovery Is Crucial in the Evaluation of New Macromolecular Cryoprotectants.Biomacromolecules · 2020Article
- Polyampholytes as Emerging Macromolecular Cryoprotectants.Biomacromolecules · 2020Review
- Extracellular Antifreeze Protein Significantly Enhances the Cryopreservation of Cell Monolayers.Biomacromolecules · 2019Article
- The ice recrystallization inhibitor polyvinyl alcohol does not improve platelet cryopreservation.Transfusion · 2019Article
- Synthetically Scalable Poly(ampholyte) Which Dramatically Enhances Cellular Cryopreservation.Biomacromolecules · 2019Article
- Multivalent Presentation of Ice Recrystallization Inhibiting Polymers on Nanoparticles Retains Activity.Langmuir : the ACS journal of surfaces and colloids · 2019Article
- Ice-recrystallization inhibiting polymers protect proteins against freeze-stress and enable glycerol-free cryostorage.Materials horizons · 2019Article
- Ice Recrystallization Inhibiting Polymers Enable Glycerol-Free Cryopreservation of Microorganisms.Biomacromolecules · 2018Article
- Facially Amphipathic Glycopolymers Inhibit Ice Recrystallization.Journal of the American Chemical Society · 2018Article
- Synthesis of Degradable Poly(vinyl alcohol) by Radical Ring-Opening Copolymerization and Ice Recrystallization Inhibition Activity.ACS macro letters · 2017Article
- Polyproline as a Minimal Antifreeze Protein Mimic That Enhances the Cryopreservation of Cell Monolayers.Angewandte Chemie (International ed. in English) · 2017Article
- Polymer mimics of biomacromolecular antifreezes.Nature communications · 2017Review
- Ultra-Low Dispersity Poly(vinyl alcohol) Reveals Significant Dispersity Effects on Ice Recrystallization Inhibition Activity.ACS macro letters · 2017Article
- Antifreeze Protein Mimetic Metallohelices with Potent Ice Recrystallization Inhibition Activity.Journal of the American Chemical Society · 2017Article
- Regioregular Alternating Polyampholytes Have Enhanced Biomimetic Ice Recrystallization Activity Compared to Random Copolymers and the Role of Side Chain versus Main Chain Hydrophobicity.Biomacromolecules · 2017Article
- Influence of Block Copolymerization on the Antifreeze Protein Mimetic Ice Recrystallization Inhibition Activity of Poly(vinyl alcohol).Biomacromolecules · 2016Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
5 authors at 3 institutions in 2 countries.
Funding
Abstract
Cell cryopreservation is an essential tool in modern biotechnology and medicine. The ability to freeze, store and distribute materials underpins basic cell biology and enables storage of donor cells needed for transplantation and regenerative medicine. However, many cell types do not survive freezing and the current state-of-the-art involves the addition of significant amounts of organic solvents as cryoprotectants, which themselves can be cytotoxic, or simply interfere with assays. A key cause of cell death in cryopreservation is ice recrystallization (growth), which primarily occurs during thawing. Here it is demonstrated that the addition of ice recrystalization inhibiting polymers to solutions containing low (non vitrifying) concentrations of DMSO enhance cell recovery rates by up to 75%. Cell functionality is also demonstrated using a placental cell line, and enhanced cryopreservation of primary rat hepatocytes is additionally shown. The crucial role of the polymers architecture (chain length) is shown, with shorter polymers being more effective than longer ones.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.