ArticleScience advances2016
Shape remodeling and blebbing of active cytoskeletal vesicles.
Article in Science advances, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 43 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
43 citing papers in PubMed, 108 citations in OpenAlex.
- Reconstitution of actomyosin networks in cell-sized liposomes dissects distinct mechanisms of membrane blebbing and symmetry breaking.Science advances · 2026Article
- Extracellular Vesicle Release Within Energetic and Quantitative Constraints: Implications for Function.Journal of extracellular vesicles · 2026Article
- Bottom-Up Coacervate-Based Artificial Cells: Integrating Cellular Hallmarks into Complex Life-Like Systems.Angewandte Chemie (International ed. in English) · 2026Review
- Lipid composition effects on the number and size of liposomes formed by the inverted emulsion method.Biophysical journal · 2026Article
- Force Transmission by Minimal Focal Adhesion Complexes Induces Synthetic Cell Deformation.ACS synthetic biology · 2026Article
- Dynamic shape remodeling of vesicles by internal active filaments.Biophysical journal · 2025Article
- A Microfluidic Platform for Actin-Based Membrane Remodeling Reveals the Stabilizing Role of Branched Actin Networks on Lipid Microdomains.Small science · 2025Article
- Nuclear Assembly in Giant Unilamellar Vesicles Encapsulating Xenopus Egg Extract.Small (Weinheim an der Bergstrasse, Germany) · 2025Article
- Reconstituted systems for studying the architecture and dynamics of actin networks.The Biochemical journal · 2025Review
- Reconstitution of actomyosin networks in cell-sized liposomes reveals distinct mechanical roles of cytoskeletal organization in membrane shape remodeling.bioRxiv : the preprint server for biology · 2025Article
- Filament transport supports contractile steady states of actin networks.bioRxiv : the preprint server for biology · 2025Article
- Active membrane deformations of a minimal synthetic cell.Nature physics · 2025Article
- Benefits and challenges of reconstituting the actin cortex.Cytoskeleton (Hoboken, N.J.) · 2024Review
- Mechanical power is maximized during contractile ring-like formation in a biomimetic dividing cell model.Nature communications · 2024Article
- Article
- Supramolecular fibrillation in coacervates and other confined systems towards biomimetic function.Communications chemistry · 2024Review
- Composite branched and linear F-actin maximize myosin-induced membrane shape changes in a biomimetic cell model.Communications biology · 2024Article
- How cytoskeletal crosstalk makes cells move: Bridging cell-free and cell studies.Biophysics reviews · 2024Review
- Visualizing Actin Packing and the Effects of Actin Attachment on Lipid Membrane Viscosity Using Molecular Rotors.JACS Au · 2024Article
- Protocells Capable of Generating a Cytoskeleton-like Structure from intracellular Membrane-active Artificial Organelles.Advanced functional materials · 2023Article
Corrections and comments
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Authors and funding
7 authors at 4 institutions in 3 countries.
Funding
Abstract
Morphological transformations of living cells, such as shape adaptation to external stimuli, blebbing, invagination, or tethering, result from an intricate interplay between the plasma membrane and its underlying cytoskeleton, where molecular motors generate forces. Cellular complexity defies a clear identification of the competing processes that lead to such a rich phenomenology. In a synthetic biology approach, designing a cell-like model assembled from a minimal set of purified building blocks would allow the control of all relevant parameters. We reconstruct actomyosin vesicles in which the coupling of the cytoskeleton to the membrane, the topology of the cytoskeletal network, and the contractile activity can all be precisely controlled and tuned. We demonstrate that tension generation of an encapsulated active actomyosin network suffices for global shape transformation of cell-sized lipid vesicles, which are reminiscent of morphological adaptations in living cells. The observed polymorphism of our cell-like model, such as blebbing, tether extrusion, or faceted shapes, can be qualitatively explained by the protein concentration dependencies and a force balance, taking into account the membrane tension, the density of anchoring points between the membrane and the actin network, and the forces exerted by molecular motors in the actin network. The identification of the physical mechanisms for shape transformations of active cytoskeletal vesicles sets a conceptual and quantitative benchmark for the further exploration of the adaptation mechanisms of cells.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.