ArticleCurrent protocols in molecular biology2016
Overview of Post Cohen-Boyer Methods for Single Segment Cloning and for Multisegment DNA Assembly.
Article in Current protocols in molecular biology, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 10 papers.
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Who cites it
10 citing papers in PubMed, 24 citations in OpenAlex.
- The plant Golden Gate toolkit: compatibility, interoperability, and harmonized assembly in plant synthetic biology.Plant methods · 2026Review
- Directed mutagenesis of large multi-subunit protein complexes by plasmid sub-fragmentation.Scientific reports · 2026Article
- TLTC, a T5 exonuclease-mediated low-temperature DNA cloning method.Frontiers in bioengineering and biotechnology · 2023Article
- Efficient golden gate assembly of DNA constructs for single molecule force spectroscopy and imaging.Nucleic acids research · 2022Article
- A Golden-Gate Based Cloning Toolkit to Build Violacein Pathway Libraries inACS synthetic biology · 2021Article
- Article
- A toolkit for DNA assembly, genome engineering and multicolor imaging forTranslational medicine of aging · 2018Article
- A Bacterial Adenylate Cyclase-Based Two-Hybrid System Compatible with GatewayMethods in molecular biology (Clifton, N.J.) · 2018Article
- Golden Gate Assembly system dedicated to complex pathway manipulation in Yarrowia lipolytica.Microbial biotechnology · 2017Article
- Using measures of single-cell physiology and physiological state to understand organismic aging.Aging cell · 2016Review
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
Abstract
In 1973, Cohen and coworkers published a foundational paper describing the cloning of DNA fragments into plasmid vectors. In it, they used DNA segments made by digestion with restriction enzymes and joined these in vitro with DNA ligase. These methods established working recombinant DNA technology and enabled the immediate start of the biotechnology industry. Since then, "classical" recombinant DNA technology using restriction enzymes and DNA ligase has matured. At the same time, researchers have developed numerous ways to generate large, complex, multisegment DNA constructions that offer advantages over classical techniques. Here, we provide an overview of "post-Cohen-Boyer" techniques used for cloning single segments into vectors (T/A, Topo cloning, Gateway and Recombineering) and for multisegment DNA assembly (BioBricks, Golden Gate, Gibson, yeast homologous recombination in vivo, and ligase cycling reaction). We compare and contrast these methods and also discuss issues that researchers should consider before choosing a particular multisegment DNA assembly method. © 2016 by John Wiley & Sons, Inc.
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