ArticleDevelopment (Cambridge, England)2016
Quantitative imaging of lipids in live mouse oocytes and early embryos using CARS microscopy.
Article in Development (Cambridge, England), 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 41 papers.
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Who cites it
41 citing papers in PubMed, 79 citations in OpenAlex.
- Metabolic hubs in reproduction: The regulatory network of lipid droplets in gamete and embryo physiology (Review).International journal of molecular medicine · 2026Review
- Advancing Label-Free Imaging Through CARS Microscopy: From Signal Formation to Biological Interpretation.International journal of molecular sciences · 2026Review
- Micro magnetic resonance spectroscopy for noninvasive metabolic screening of mammalian embryos and oocytes.Proceedings of the National Academy of Sciences of the United States of America · 2025Article
- Metabolic regulation of key developmental events during mammalian embryogenesis.Nature cell biology · 2025Review
- Lipid Metabolic Heterogeneity during Early Embryogenesis Revealed by Hyper-3D Stimulated Raman Imaging.Chemical & biomedical imaging · 2025Article
- Non-invasive applications of Raman spectroscopy in assisted reproduction.Frontiers in endocrinology · 2025Review
- Long-chain fatty acid β-oxidation regulates embryonic development by H3K18 acetylation in mice.Frontiers in cell and developmental biology · 2025Article
- Label-free, high-throughput holographic imaging to evaluate mammalian gametes and embryos†.Biology of reproduction · 2024Review
- Low-input lipidomics reveals lipid metabolism remodelling during early mammalian embryo development.Nature cell biology · 2024Article
- How great thou ART: biomechanical properties of oocytes and embryos as indicators of quality in assisted reproductive technologies.Frontiers in cell and developmental biology · 2024Review
- KLF4 facilitates chromatin accessibility remodeling in porcine early embryos.Science China. Life sciences · 2024Article
- Drosophila embryos allocate lipid droplets to specific lineages to ensure punctual development and redox homeostasis.PLoS genetics · 2023Article
- Stimulated Raman Scattering Imaging Sheds New Light on Lipid Droplet Biology.The journal of physical chemistry. B · 2023Article
- Scd1 Deficiency in Early Embryos Affects Blastocyst ICM Formation through RPs-Mdm2-p53 Pathway.International journal of molecular sciences · 2023Article
- Species and embryo genome origin affect lipid droplets in preimplantation embryos.Frontiers in cell and developmental biology · 2023Article
- Nile Red and BODIPY Staining of Lipid Droplets in Mouse Oocytes and Embryos.Methods in molecular biology (Clifton, N.J.) · 2023Article
- Correlative Raman-Electron-Light (CREL) Microscopy Analysis of Lipid Droplets in Melanoma Cancer Stem Cells.Biosensors · 2022Article
- Plin2-mediated lipid droplet mobilization accelerates exit from pluripotency by lipidomic remodeling and histone acetylation.Cell death and differentiation · 2022Article
- Dynamic enlargement and mobilization of lipid droplets in pluripotent cells coordinate morphogenesis during mouse peri-implantation development.Nature communications · 2022Article
- Dissecting lipid droplet biology with coherent Raman scattering microscopy.Journal of cell science · 2022Review
Corrections and comments
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Authors and funding
7 authors at 1 institution in 1 country.
Funding
Abstract
Mammalian oocytes contain lipid droplets that are a store of fatty acids, whose metabolism plays a substantial role in pre-implantation development. Fluorescent staining has previously been used to image lipid droplets in mammalian oocytes and embryos, but this method is not quantitative and often incompatible with live cell imaging and subsequent development. Here we have applied chemically specific, label-free coherent anti-Stokes Raman scattering (CARS) microscopy to mouse oocytes and pre-implantation embryos. We show that CARS imaging can quantify the size, number and spatial distribution of lipid droplets in living mouse oocytes and embryos up to the blastocyst stage. Notably, it can be used in a way that does not compromise oocyte maturation or embryo development. We have also correlated CARS with two-photon fluorescence microscopy simultaneously acquired using fluorescent lipid probes on fixed samples, and found only a partial degree of correlation, depending on the lipid probe, clearly exemplifying the limitation of lipid labelling. In addition, we show that differences in the chemical composition of lipid droplets in living oocytes matured in media supplemented with different saturated and unsaturated fatty acids can be detected using CARS hyperspectral imaging. These results demonstrate that CARS microscopy provides a novel non-invasive method of quantifying lipid content, type and spatial distribution with sub-micron resolution in living mammalian oocytes and embryos.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.