ArticleScience (New York, N.Y.)2016
Femtosecond structural dynamics drives the trans/cis isomerization in photoactive yellow protein.
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Article in Science (New York, N.Y.), 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 180 papers.
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180citing papers in PubMed
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180 citing papers in PubMed.
- Analysing the significance of small conformational changes and low occupancy states in serial crystallographic data.FEBS open bio · 2026Article
- Refinement of amino-acid conformation vs. difference density maps in time-resolved serial femtosecond crystallography data analysis.FEBS open bio · 2026Article
- A simple and practical approach for protein serial crystallography using grease matrix and large-area support film.Journal of synchrotron radiation · 2026Article
- From Static Structures to Molecular Dynamics: Emerging Directions in X-Ray and Electron Materials Characterization.Materials (Basel, Switzerland) · 2026Review
- Few-femtosecond photoelectron spectroscopy reveals fundamental photodynamics of the carbon-carbon double bond in ethylene.Nature communications · 2026Article
- Theoretical Design of All-Optical Universal Logic via Multistage FRET-STED Energy Cascades in Fluorescent Protein Nanoclusters.ACS omega · 2026Article
- Liquid-Microjet Photoelectron Spectroscopy of the Photoactive Yellow Protein Chromophore in Aqueous Solution.The journal of physical chemistry. A · 2026Article
- A bacteriophytochrome Pr/Pfr heterodimer studied through single-particle time-resolved cryo-electron microscopy.Communications chemistry · 2026Article
- Advances in Crystallography and Cryo-Electron Microscopy for the Study of Membrane Proteins.Advances in experimental medicine and biology · 2026Review
- Hot Ground State Cooling Following Ultrafast Photoisomerization: Time-Resolved Infrared Spectroscopy.The journal of physical chemistry. B · 2025Article
- Real-time capture of domain movements during copper amine oxidase catalysis by mix-and-inject serial crystallography.Nature communications · 2025Article
- Microsecond Time-Resolved Cryo-EM Based on Jet Vitrification.bioRxiv : the preprint server for biology · 2025Article
- Sensitive detection of structural dynamics using a statistical framework for comparative crystallography.Science advances · 2025Article
- Denoising and iterative phase recovery reveal low-occupancy populations in protein crystals.Communications biology · 2025Article
- Sample delivery methods for protein X-ray crystallography with a special focus on sample consumption.Nature communications · 2025Review
- Ultrafast, remote-controlled protonation reaction enables structural changes in a phytochrome.Science advances · 2025Article
- Direct Observation of the Exciton-Polaron in Single CsPbBrACS nano · 2025Article
- Computational study of terahertz-driven controllable molecular isomerization.Nature communications · 2025Article
- Advancing time-resolved structural biology: latest strategies in cryo-EM and X-ray crystallography.Nature methods · 2025Review
- Observation of early events in the photoactivation of Myxobacterial phytochrome using time-resolved serial femtosecond crystallography.Communications chemistry · 2025Article
120 more citing papers are in PubMed but not listed here.
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Authors and funding
44 authors.
Kanupriya PandeDepartment of Physics, University of Wisconsin-Milwaukee, Milwaukee, WI 53211, USA. Center for Free Electron Laser Science, Deutsches Elektronen Synchrotron, Notkestrasse 85, 22607 Hamburg, Germany.
Christopher D M HutchisonFaculty of Natural Sciences, Department of Life Sciences, Imperial College, London SW7 2AZ, UK.
Gerrit GroenhofNanoscience Center and Department of Chemistry, University of Jyväskylä, Post Office Box 35, 40014 Jyväskylä, Finland.
Andy AquilaLinac Coherent Light Source, SLAC National Accelerator Laboratory, Sand Hill Road, Menlo Park, CA 94025, USA.
Josef S RobinsonLinac Coherent Light Source, SLAC National Accelerator Laboratory, Sand Hill Road, Menlo Park, CA 94025, USA.
Jason TenboerDepartment of Physics, University of Wisconsin-Milwaukee, Milwaukee, WI 53211, USA.
Shibom BasuSchool of Molecular Sciences and Biodesign Center for Applied Structural Discovery, Arizona State University, Tempe, AZ 85287, USA.
Sébastien BoutetLinac Coherent Light Source, SLAC National Accelerator Laboratory, Sand Hill Road, Menlo Park, CA 94025, USA.
Daniel P DePonteLinac Coherent Light Source, SLAC National Accelerator Laboratory, Sand Hill Road, Menlo Park, CA 94025, USA.
Mengning LiangLinac Coherent Light Source, SLAC National Accelerator Laboratory, Sand Hill Road, Menlo Park, CA 94025, USA.
Thomas A WhiteCenter for Free Electron Laser Science, Deutsches Elektronen Synchrotron, Notkestrasse 85, 22607 Hamburg, Germany.
Nadia A ZatsepinDepartment of Physics, Arizona State University, Tempe, AZ 85287, USA.
Oleksandr YefanovCenter for Free Electron Laser Science, Deutsches Elektronen Synchrotron, Notkestrasse 85, 22607 Hamburg, Germany.
Dmitry MorozovNanoscience Center and Department of Chemistry, University of Jyväskylä, Post Office Box 35, 40014 Jyväskylä, Finland.
Dominik OberthuerCenter for Free Electron Laser Science, Deutsches Elektronen Synchrotron, Notkestrasse 85, 22607 Hamburg, Germany.
Cornelius GatiCenter for Free Electron Laser Science, Deutsches Elektronen Synchrotron, Notkestrasse 85, 22607 Hamburg, Germany.
Ganesh SubramanianDepartment of Physics, Arizona State University, Tempe, AZ 85287, USA.
Daniel JamesDepartment of Physics, Arizona State University, Tempe, AZ 85287, USA.
Yun ZhaoDepartment of Physics, Arizona State University, Tempe, AZ 85287, USA.
Jake KoralekLinac Coherent Light Source, SLAC National Accelerator Laboratory, Sand Hill Road, Menlo Park, CA 94025, USA.
Jennifer BrayshawDepartment of Physics, University of Wisconsin-Milwaukee, Milwaukee, WI 53211, USA.
Christopher KupitzDepartment of Physics, University of Wisconsin-Milwaukee, Milwaukee, WI 53211, USA.
Chelsie ConradSchool of Molecular Sciences and Biodesign Center for Applied Structural Discovery, Arizona State University, Tempe, AZ 85287, USA.
Shatabdi Roy-ChowdhurySchool of Molecular Sciences and Biodesign Center for Applied Structural Discovery, Arizona State University, Tempe, AZ 85287, USA.
Jesse D CoeSchool of Molecular Sciences and Biodesign Center for Applied Structural Discovery, Arizona State University, Tempe, AZ 85287, USA.
Markus MetzCenter for Free Electron Laser Science, Deutsches Elektronen Synchrotron, Notkestrasse 85, 22607 Hamburg, Germany.
Paulraj Lourdu XavierCenter for Free Electron Laser Science, Deutsches Elektronen Synchrotron, Notkestrasse 85, 22607 Hamburg, Germany. IMPRS-UFAST, Max Planck Institute for Structure and Dynamics of Matter, Luruper Chaussee 149, 22761 Hamburg, Germany.
Thomas D GrantHauptman-Woodward Institute, State University of New York at Buffalo, 700 Ellicott Street, Buffalo, NY 14203, USA.
Jason E KoglinLinac Coherent Light Source, SLAC National Accelerator Laboratory, Sand Hill Road, Menlo Park, CA 94025, USA.
Gihan KetawalaSchool of Molecular Sciences and Biodesign Center for Applied Structural Discovery, Arizona State University, Tempe, AZ 85287, USA.
Raimund FrommeSchool of Molecular Sciences and Biodesign Center for Applied Structural Discovery, Arizona State University, Tempe, AZ 85287, USA.
Vukica ŠrajerCenter for Advanced Radiation Sources, University of Chicago, Chicago, IL 60637, USA.
Robert HenningCenter for Advanced Radiation Sources, University of Chicago, Chicago, IL 60637, USA.
John C H SpenceDepartment of Physics, Arizona State University, Tempe, AZ 85287, USA.
Abbas OurmazdDepartment of Physics, University of Wisconsin-Milwaukee, Milwaukee, WI 53211, USA.
Peter SchwanderDepartment of Physics, University of Wisconsin-Milwaukee, Milwaukee, WI 53211, USA.
Uwe WeierstallDepartment of Physics, Arizona State University, Tempe, AZ 85287, USA.
Matthias FrankLawrence Livermore National Laboratory, Livermore, CA 94550, USA.
Petra FrommeSchool of Molecular Sciences and Biodesign Center for Applied Structural Discovery, Arizona State University, Tempe, AZ 85287, USA.
Anton BartyCenter for Free Electron Laser Science, Deutsches Elektronen Synchrotron, Notkestrasse 85, 22607 Hamburg, Germany.
Henry N ChapmanCenter for Free Electron Laser Science, Deutsches Elektronen Synchrotron, Notkestrasse 85, 22607 Hamburg, Germany. Center for Ultrafast Imaging, University of Hamburg, Luruper Chaussee 149, 22761 Hamburg, Germany.
Keith MoffatCenter for Advanced Radiation Sources, University of Chicago, Chicago, IL 60637, USA. Department of Biochemistry and Molecular Biology and Institute for Biophysical Dynamics, University of Chicago, Chicago, IL 60637, USA.
Jasper J van ThorFaculty of Natural Sciences, Department of Life Sciences, Imperial College, London SW7 2AZ, UK.
Marius SchmidtDepartment of Physics, University of Wisconsin-Milwaukee, Milwaukee, WI 53211, USA.
Funding
ZWITTERION SURFACTANT DDMAB FOR IMPROV STABIL OF PROTEINP41RR001209 · NCRR · STANFORD UNIVERSITY · PI GONZALEZ, ANA M · 1985 to 2011
$38.9MX-ray Absorption Spectroscopy (XAS) pp.711-759P41GM103393 · NIGMS · STANFORD UNIVERSITY · PI HODGSON, KEITH O · 2012 to 2019
$30.6MX-RAY STUDY OF HORMONE-RECEPTOR AND PROTEIN-ANTIBODY COMPLEXESP41RR007707 · NCRR · UNIVERSITY OF CHICAGO · PI YANG, XIANGZHONG · 2002 to 2011
$17.3MFemtosecond nano-crystallography of membrane proteinsR01GM095583 · NIGMS · ARIZONA STATE UNIVERSITY-TEMPE CAMPUS · PI FROMME, PETRA · 2010 to 2023
$4.3MStructures, Dynamics and Signaling Mechanisms of Modular PhotoreceptorsR01EY024363 · NEI · UNIVERSITY OF ILLINOIS AT CHICAGO · PI YANG, XIAOJING · 2014 to 2021
$2.9MBioCARS: A Synchrotron Structure Biology ResourceR24GM111072 · NIGMS · UNIVERSITY OF CHICAGO · PI MOFFAT, JOHN KEITH · 2013 to 2016
$2.3MNCRR NIH HHS P41 RR001209NCRR NIH HHS P41RR001209NCRR NIH HHS P41 RR007707NEI NIH HHS R01 EY024363NEI NIH HHS R01EY024363NIGMS NIH HHS P41 GM103393NIGMS NIH HHS P41GM103393NIGMS NIH HHS R01 GM095583NIGMS NIH HHS R01GM095583NIGMS NIH HHS R24 GM111072NIGMS NIH HHS R24GM111072
Abstract
A variety of organisms have evolved mechanisms to detect and respond to light, in which the response is mediated by protein structural changes after photon absorption. The initial step is often the photoisomerization of a conjugated chromophore. Isomerization occurs on ultrafast time scales and is substantially influenced by the chromophore environment. Here we identify structural changes associated with the earliest steps in the trans-to-cis isomerization of the chromophore in photoactive yellow protein. Femtosecond hard x-ray pulses emitted by the Linac Coherent Light Source were used to conduct time-resolved serial femtosecond crystallography on photoactive yellow protein microcrystals over a time range from 100 femtoseconds to 3 picoseconds to determine the structural dynamics of the photoisomerization reaction.
Indexed as
Photochemical ProcessesBacterial ProteinsCrystallographyIsomerismLightPhotonsPhotoreceptors, MicrobialProtein ConformationTime FactorsBacterial Proteinsphotoactive yellow protein, BacteriaPhotoreceptors, Microbial
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