Evidence map›Paper›PMID 27151220›Full record

ArticleThe Journal of biological chemistry2016

The N Terminus of the Vaccinia Virus Protein F1L Is an Intrinsically Unstructured Region That Is Not Involved in Apoptosis Regulation.

Sofia Caria, Bevan Marshall, Robyn-Lee Burton, Stephanie Campbell, Delara Pantaki-Eimany, Christine J Hawkins, Michele Barry, Marc Kvansakul

Open access · hybridAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in The Journal of biological chemistry, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers.

0numbers the graph read from it
0cells of the map it votes in
13citing papers in PubMed
1.4field-weighted citation impact, top 18% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

13 citing papers in PubMed, 16 citations in OpenAlex.

  1. Article
  2. Review
  3. Review
  4. Contribution of yeast models to virus research.Applied microbiology and biotechnology · 2021
    Review
  5. Poxviral Strategies to Overcome Host Cell Apoptosis.Pathogens (Basel, Switzerland) · 2020
    Review
  6. Review
  7. Article
  8. Article
  9. Article
  10. Review
  11. Review
  12. Article
  13. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

8 authors at 2 institutions in 2 countries.

Sofia CariaFrom the La Trobe Institute for Molecular Science, Department of Biochemistry and Genetics, La Trobe University, Victoria 3086, Australia and.
Bevan MarshallFrom the La Trobe Institute for Molecular Science, Department of Biochemistry and Genetics, La Trobe University, Victoria 3086, Australia and.
Robyn-Lee BurtonLi Ka Shing Institute for Virology, Department of Medical Microbiology and Immunology, University of Alberta, Edmonton, Alberta T6G 2S2, Canada.
Stephanie CampbellLi Ka Shing Institute for Virology, Department of Medical Microbiology and Immunology, University of Alberta, Edmonton, Alberta T6G 2S2, Canada.
Delara Pantaki-EimanyFrom the La Trobe Institute for Molecular Science, Department of Biochemistry and Genetics, La Trobe University, Victoria 3086, Australia and.
Christine J HawkinsFrom the La Trobe Institute for Molecular Science, Department of Biochemistry and Genetics, La Trobe University, Victoria 3086, Australia and.
Michele BarryLi Ka Shing Institute for Virology, Department of Medical Microbiology and Immunology, University of Alberta, Edmonton, Alberta T6G 2S2, Canada micheleb@ualberta.ca.
Marc KvansakulFrom the La Trobe Institute for Molecular Science, Department of Biochemistry and Genetics, La Trobe University, Victoria 3086, Australia and m.kvansakul@latrobe.edu.au.ORCID 0000-0003-2639-2498
La Trobe University · AUUniversity of Alberta · CA

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Subversion of host cell apoptotic responses is a prominent feature of viral immune evasion strategies to prevent premature clearance of infected cells. Numerous poxviruses encode structural and functional homologs of the Bcl-2 family of proteins, and vaccinia virus harbors antiapoptotic F1L that potently inhibits the mitochondrial apoptotic checkpoint. Recently F1L has been assigned a caspase-9 inhibitory function attributed to an N-terminal α helical region of F1L spanning residues 1-15 (1) preceding the domain-swapped Bcl-2-like domains. Using a reconstituted caspase inhibition assay in yeast we found that unlike AcP35, a well characterized caspase-9 inhibitor from the insect virus Autographa californica multiple nucleopolyhedrovirus, F1L does not prevent caspase-9-mediated yeast cell death. Furthermore, we found that deletion of the F1L N-terminal region does not impede F1L antiapoptotic activity in the context of a viral infection. Solution analysis of the F1L N-terminal regions using small angle x-ray scattering indicates that the region of F1L spanning residues 1-50 located N-terminally from the Bcl-2 fold is an intrinsically unstructured region. We conclude that the N terminus of F1L is not involved in apoptosis inhibition and may act as a regulatory element in other signaling pathways in a manner reminiscent of other unstructured regulatory elements commonly found in mammalian prosurvival Bcl-2 members including Bcl-xL and Mcl-1.

Indexed as

ApoptosisAmino Acid SequenceHEK293 CellsHeLa CellsHumansModels, MolecularProtein ConformationProto-Oncogene Proteins c-bcl-2Scattering, Small AngleSequence AlignmentVacciniaVaccinia virusViral ProteinsX-Ray DiffractionF1L protein, vaccinia virusProto-Oncogene Proteins c-bcl-2Viral ProteinsapoptosisB-cell lymphoma 2 (Bcl-2) familycaspasespoxvirusprotein structuresmall angle x-ray scattering (SAXS)vaccinia virus

Identifiers

PMID27151220
PMCPMC4938181
OpenAlexW2345423652

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.