Evidence map›Paper›PMID 27151208›Full record

ArticleDevelopmental biology2016

Bhlhb5::flpo allele uncovers a requirement for Bhlhb5 for the development of the dorsal cochlear nucleus.

Xiaoyun Cai, Adam P Kardon, Lindsey M Snyder, Marissa S Kuzirian, Sam Minestro, Luiza de Souza, Maria E Rubio, Stephen M Maricich, Sarah E Ross

Open access · hybridAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Developmental biology, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 12 papers.

0numbers the graph read from it
0cells of the map it votes in
12citing papers in PubMed
1.2field-weighted citation impact, top 25% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

12 citing papers in PubMed, 19 citations in OpenAlex.

  1. Journal of medical genetics · 2026
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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

9 authors at 1 institution in 1 country.

Xiaoyun CaiDepartment of Neurobiology, University of Pittsburgh, 200 Lothrop St., Pittsburgh, PA 15213, USA; The Pittsburgh Center for Pain Research, University of Pittsburgh, 200 Lothrop St., Pittsburgh, PA 15213, USA.
Adam P KardonDepartment of Neurobiology, University of Pittsburgh, 200 Lothrop St., Pittsburgh, PA 15213, USA; The Pittsburgh Center for Pain Research, University of Pittsburgh, 200 Lothrop St., Pittsburgh, PA 15213, USA.
Lindsey M SnyderDepartment of Neurobiology, University of Pittsburgh, 200 Lothrop St., Pittsburgh, PA 15213, USA; The Pittsburgh Center for Pain Research, University of Pittsburgh, 200 Lothrop St., Pittsburgh, PA 15213, USA.
Marissa S KuzirianDepartment of Neurobiology, University of Pittsburgh, 200 Lothrop St., Pittsburgh, PA 15213, USA; The Pittsburgh Center for Pain Research, University of Pittsburgh, 200 Lothrop St., Pittsburgh, PA 15213, USA.
Sam MinestroDepartment of Neurobiology, University of Pittsburgh, 200 Lothrop St., Pittsburgh, PA 15213, USA.
Luiza de SouzaDepartment of Neurobiology, University of Pittsburgh, 200 Lothrop St., Pittsburgh, PA 15213, USA.
Maria E RubioDepartment of Neurobiology, University of Pittsburgh, 200 Lothrop St., Pittsburgh, PA 15213, USA; Department of Otolaryngology, University of Pittsburgh, Pittsburgh, PA, USA.
Stephen M MaricichRichard King Mellon Institute for Pediatric Research, Department of Pediatrics, University of Pittsburgh, Pittsburgh, PA, USA; Children's Hospital of Pittsburgh of UPMC, Pittsburgh, PA, USA.
Sarah E RossDepartment of Neurobiology, University of Pittsburgh, 200 Lothrop St., Pittsburgh, PA 15213, USA; The Pittsburgh Center for Pain Research, University of Pittsburgh, 200 Lothrop St., Pittsburgh, PA 15213, USA; Department of Anesthesiology, University of Pittsburgh, 200 Lothrop St., Pittsburgh, PA 15213, USA. Electronic address: saross@pitt.edu.
University of Pittsburgh · US

Funding

SYNAPTIC MECHANISMS IN HIPPOCAMPAL EPILEPTOGENESISP30HD018655 · NICHD · CHILDREN'S HOSPITAL BOSTON · PI POMEROY, SCOTT LOREN · 1985 to 2015
$26.2M
Investigating the Neural Circuits of ItchR01AR063772 · NIAMS · UNIVERSITY OF PITTSBURGH AT PITTSBURGH · PI H Richard Koerber, Sarah Elizabeth Ross · 2013 to 2026
$6.9M
Mechanisms of hypersensitivity to sound-induced cochlear damageR01DC013048 · NIDCD · UNIVERSITY OF PITTSBURGH AT PITTSBURGH · PI Maria-Eulalia Rubio-Valero · 2013 to 2026
$6.8M
Using dual intersectional genetics to understand and modulate itchR21AR064445 · NIAMS · UNIVERSITY OF PITTSBURGH AT PITTSBURGH · PI ROSS, SARAH ELIZABETH · 2013 to 2014
$358k
Understanding a specific microcircuit that integrates somatosensory information in the dorsal spinal cordF31NS092146 · NINDS · UNIVERSITY OF PITTSBURGH AT PITTSBURGH · PI SNYDER, LINDSEY M · 2015 to 2016
$83k
NIAMS NIH HHS R01 AR063772NIAMS NIH HHS R21 AR064445NICHD NIH HHS P30 HD018655NIDCD NIH HHS R01 DC013048NINDS NIH HHS F31 NS092146
6 · The paper itself

Abstract

Auditory information is initially processed in the cochlear nuclei before being relayed to the brain. The cochlear nuclei are subdivided into dorsal, anterior ventral, and posterior ventral domains, each containing several subtypes of neurons that are thought to play discrete roles in the processing of sound. However, the ontogeny of these neurons is poorly understood, and this gap in knowledge hampers efforts to understand the basic neural circuitry of this nucleus. Here, we reveal that Bhlhb5 is expressed in both excitatory (unipolar brush cells) and inhibitory neurons (cartwheel cells) of the DCN during development. To gain genetic access to Bhlhb5-expressing neurons in the DCN, we generated a Bhlhb5::flpo knockin allele. Using an intersectional genetic strategy, we labeled cartwheel cells, thereby providing proof of concept that subpopulations of Bhlhb5-expressing neurons can be genetically targeted. Moreover, fate-mapping experiments using this allele revealed that Bhlhb5 is required for the proper development of the DCN, since mice lacking Bhlhb5 showed a dramatically diminished number of neurons, including unipolar brush and cartwheel cells. Intriguingly, the Bhlhb5::flpo allele also genetically labels numerous other regions of the nervous system that process sensory input, including the dorsal horn, the retina, and the nucleus of the lateral olfactory tract, hinting at a more general role for Bhlhb5 in the development of neurons that mediate sensory integration.

Indexed as

AllelesAnimalsBasic Helix-Loop-Helix ProteinsCell CountCell LineageCochlear NucleusGene Expression Regulation, DevelopmentalGene Knock-In TechniquesLuminescent ProteinsMiceMice, KnockoutOlfactory BulbPAX6 Transcription FactorPosterior Horn CellsRetinaSensory Receptor CellsBasic Helix-Loop-Helix ProteinsBhlhe22 protein, mouseLuminescent ProteinsPAX6 Transcription FactorBhlhb5Bhlhe22Cre recombinaseDorsal cochlear nucleusFlp recombinase

Identifiers

PMID27151208
PMCPMC4930277
OpenAlexW2346844476

What OpenQuestion holds

Textmetadata
LicenceCC BY-NC-ND
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.