ArticleScientific reports2016
Wheat seed embryo excision enables the creation of axenic seedlings and Koch's postulates testing of putative bacterial endophytes.
Article in Scientific reports, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 30 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
30 citing papers in PubMed.
- Successive cultivation under drought selects for specific microbiome members in the wheat rhizosphere.FEMS microbiology ecology · 2026Article
- Spatial Transcriptomics of Developing Wheat Seed Reveals Concentric Gene Expression Zones and Subgenome Biased Expression of Key Genes.Plant biotechnology journal · 2025Article
- Intergenerational and horizontal transmission of wheat endophytic fungi.Scientific reports · 2025Article
- Article
- Seed-mediated vertical transmission of Pantoea core endophytes.The ISME journal · 2025Article
- Agroecology-based assembly and function of endophytic bacteria in seeds ofFrontiers in microbiology · 2025Article
- Article
- Wheat fungal endophyte communities are inseparable from the host and influence plant development.mBio · 2024Article
- Endophytes in Agriculture: Potential to Improve Yields and Tolerances of Agricultural Crops.Microorganisms · 2023Review
- Australian native Glycine clandestina seed microbiota hosts a more diverse bacterial community than the domesticated soybean Glycine max.Environmental microbiome · 2022Article
- Tailored Media Are Key to Unlocking the Diversity of Endophytic Bacteria in Distinct Compartments of Germinating Seeds.Microbiology spectrum · 2022Article
- Marine Natural Product Antimycin A Suppresses Wheat Blast Disease Caused byJournal of fungi (Basel, Switzerland) · 2022Article
- Biosynthetic Mechanisms of Secondary Metabolites Promoted by the Interaction Between Endophytes and Plant Hosts.Frontiers in microbiology · 2022Review
- Variable influences of soil and seed-associated bacterial communities on the assembly of seedling microbiomes.The ISME journal · 2021Article
- Diversity and characteristics of culturable endophytic bacteria from Passiflora edulis seeds.MicrobiologyOpen · 2021Article
- Domestication affects the composition, diversity, and co-occurrence of the cereal seed microbiota.Journal of advanced research · 2021Article
- Endophytic BacteriaBiology · 2021Article
- Wheat Microbiome: Structure, Dynamics, and Role in Improving Performance Under Stress Environments.Frontiers in microbiology · 2021Review
- Domestication Impacts the Wheat-Associated Microbiota and the Rhizosphere Colonization by Seed- and Soil-Originated Microbiomes, Across Different Fields.Frontiers in plant science · 2021Article
- Vertical Transmission of Diverse Cultivation-Recalcitrant Endophytic Bacteria Elucidated Using Watermelon Seed Embryos.Frontiers in microbiology · 2021Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
6 authors.
Funding
Abstract
Early establishment of endophytes can play a role in pathogen suppression and improve seedling development. One route for establishment of endophytes in seedlings is transmission of bacteria from the parent plant to the seedling via the seed. In wheat seeds, it is not clear whether this transmission route exists, and the identities and location of bacteria within wheat seeds are unknown. We identified bacteria in the wheat (Triticum aestivum) cv. Hereward seed environment using embryo excision to determine the location of the bacterial load. Axenic wheat seedlings obtained with this method were subsequently used to screen a putative endophyte bacterial isolate library for endophytic competency. This absence of bacteria recovered from seeds indicated low bacterial abundance and/or the presence of inhibitors. Diversity of readily culturable bacteria in seeds was low with 8 genera identified, dominated by Erwinia and Paenibacillus. We propose that anatomical restrictions in wheat limit embryo associated vertical transmission, and that bacterial load is carried in the seed coat, crease tissue and endosperm. This finding facilitates the creation of axenic wheat plants to test competency of putative endophytes and also provides a platform for endophyte competition, plant growth, and gene expression studies without an indigenous bacterial background.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.