ArticleBMC microbiology2016
In vivo gene expression in a Staphylococcus aureus prosthetic joint infection characterized by RNA sequencing and metabolomics: a pilot study.
Article in BMC microbiology, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 36 papers.
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Who cites it
36 citing papers in PubMed, 55 citations in OpenAlex.
- [18F]Fluorodeoxyglucose Positron Emission Tomography for Diagnosis and Monitoring of Acute Staphylococcus aureus Vascular Graft Infection in a Rat Model.The Journal of infectious diseases · 2026Article
- Advancing diagnostics in suspected periprosthetic joint infections using synthetic synovial fluid and microcalorimetry.Journal of bone and joint infection · 2026Article
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- Bithionol is ineffective in a mouse model of S. aureus implant-associated osteomyelitis despite potent in vitro activity.Scientific reports · 2025Article
- The role of bacterial metabolism in antimicrobial resistance.Nature reviews. Microbiology · 2025Review
- Tissue niche influences immune and metabolic profiles to Staphylococcus aureus biofilm infection.Nature communications · 2024Article
- Ecological and evolutionary mechanisms driving within-patient emergence of antimicrobial resistance.Nature reviews. Microbiology · 2024Review
- Tryptophanyl tRNA synthetase is an alternative synovial biomarker for diagnosis of septic arthritis in knee joint.Knee surgery & related research · 2024Article
- A high-throughput assay identifies molecules with antimicrobial activity against persister cells.Journal of medical microbiology · 2024Article
- Co-culturing withFrontiers in cellular and infection microbiology · 2024Article
- What's in a name? Characteristics of clinical biofilms.FEMS microbiology reviews · 2023Article
- Antibody-Drug Conjugates to Treat Bacterial Biofilms via Targeting and Extracellular Drug Release.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2023Article
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- Human Osteoblast-Conditioned Media Can InfluenceInternational journal of molecular sciences · 2022Article
- Transcriptome change of Staphylococcus aureus in infected mouse liver.Communications biology · 2022Article
- Search for a Shared Genetic or Biochemical Basis for Biofilm Tolerance to Antibiotics across Bacterial Species.Antimicrobial agents and chemotherapy · 2022Article
- Biofilm Survival Strategies in Chronic Wounds.Microorganisms · 2022Review
- The importance of understanding the infectious microenvironment.The Lancet. Infectious diseases · 2022Review
- Transcriptional Profiling of Pseudomonas aeruginosa Infections.Advances in experimental medicine and biology · 2022Review
- A novel chronic wound biofilm model sustaining coexistence of Pseudomonas aeruginosa and Staphylococcus aureus suitable for testing of antibiofilm effect of antimicrobial solutions and wound dressings.Wound repair and regeneration : official publication of the Wound Healing Society [and] the European Tissue Repair Society · 2021Article
Corrections and comments
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Authors and funding
9 authors at 3 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundStaphylococcus aureus gene expression has been sparsely studied in deep-sited infections in humans. Here, we characterized the staphylococcal transcriptome in vivo and the joint fluid metabolome in a prosthetic joint infection with an acute presentation using deep RNA sequencing and nuclear magnetic resonance spectroscopy, respectively. We compared our findings with the genome, transcriptome and metabolome of the S. aureus joint fluid isolate grown in vitro.
resultFrom the transcriptome analysis we found increased expression of siderophore synthesis genes and multiple known virulence genes. The regulatory pattern of catabolic pathway genes indicated that the bacterial infection was sustained on amino acids, glycans and nucleosides. Upregulation of fermentation genes and the presence of ethanol in joint fluid indicated severe oxygen limitation in vivo.
conclusionThis single case study highlights the capacity of combined transcriptome and metabolome analyses for elucidating the pathogenesis of prosthetic infections of major clinical importance.
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