ArticleScientific reports2016
Ultrasonic acoustic levitation for fast frame rate X-ray protein crystallography at room temperature.
Article in Scientific reports, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 23 papers.
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Who cites it
23 citing papers in PubMed.
- Evolution of macromolecular crystallography beamlines at the Swiss Light Source and SwissFEL.Journal of synchrotron radiation · 2025Article
- Semidefinite programming for manipulating acoustic traps in real time (SMART).Scientific reports · 2025Article
- Coalescence of multiple pairs of levitated droplets using dual-side phased arrays.Ultrasonics sonochemistry · 2025Article
- Contact-free magnetic resonance imaging and spectroscopy with acoustic levitation.Nature communications · 2025Article
- Biological Acoustic Levitation and Its Potential Application for Microgravity Study.Bioengineering (Basel, Switzerland) · 2025Review
- Review
- Hydrodynamic coupling melts acoustically levitated crystalline rafts.Proceedings of the National Academy of Sciences of the United States of America · 2023Article
- Airborne ultrasound pulse amplification based on acoustic resonance switching.Scientific reports · 2022Article
- Acoustic levitation and rotation of thin films and their application for room temperature protein crystallography.Scientific reports · 2022Article
- Measurement of Secondary Structure Changes in Poly-L-lysine and Lysozyme during Acoustically Levitated Single Droplet Drying Experiments by In Situ Raman Spectroscopy.Sensors (Basel, Switzerland) · 2022Article
- Dynamic Acoustic Levitator Based On Subwavelength Aperture Control.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2021Article
- Serial Crystallography for Structure-Based Drug Discovery.Trends in pharmacological sciences · 2020Review
- Current status and future opportunities for serial crystallography at MAX IV Laboratory.Journal of synchrotron radiation · 2020Article
- Coalescence Dynamics of Acoustically Levitated Droplets.Micromachines · 2020Article
- Evaporation and drying kinetics of water-NaCl dropletsRSC advances · 2020Article
- Acoustic Manipulation of Droplets under Reduced Gravity.Scientific reports · 2019Article
- Non-Contact Universal Sample Presentation for Room Temperature Macromolecular Crystallography Using Acoustic Levitation.Scientific reports · 2019Article
- Two-Dimensional Manipulation in Mid-Air Using a Single Transducer Acoustic Levitator.Micromachines · 2019Article
- Strategies for sample delivery for femtosecond crystallography.Acta crystallographica. Section D, Structural biology · 2019Review
- Acoustic tweezers for the life sciences.Nature methods · 2018Review
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Authors and funding
2 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Increasing the data acquisition rate of X-ray diffraction images for macromolecular crystals at room temperature at synchrotrons has the potential to significantly accelerate both structural analysis of biomolecules and structure-based drug developments. Using lysozyme model crystals, we demonstrated the rapid acquisition of X-ray diffraction datasets by combining a high frame rate pixel array detector with ultrasonic acoustic levitation of protein crystals in liquid droplets. The rapid spinning of the crystal within a levitating droplet ensured an efficient sampling of the reciprocal space. The datasets were processed with a program suite developed for serial femtosecond crystallography (SFX). The structure, which was solved by molecular replacement, was found to be identical to the structure obtained by the conventional oscillation method for up to a 1.8-Å resolution limit. In particular, the absence of protein crystal damage resulting from the acoustic levitation was carefully established. These results represent a key step towards a fully automated sample handling and measurement pipeline, which has promising prospects for a high acquisition rate and high sample efficiency for room temperature X-ray crystallography.
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Registered trials
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