ArticleCell reports2016
Improved Monosynaptic Neural Circuit Tracing Using Engineered Rabies Virus Glycoproteins.
Article in Cell reports, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 140 papers.
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Who cites it
140 citing papers in PubMed.
- An applicable and efficient retrograde monosynaptic circuit mapping tool for larval zebrafish.eLife · 2026Article
- Rabies Virus Glycoprotein G Variation Is Associated with Neurovirulence and Reduced Vamp2 Abundance in a Mouse Model of Direct CNS Infection.Veterinary sciences · 2026Article
- A male-biased hypothalamic circuit modulates postmating sleep suppression in mice.Science advances · 2026Article
- Barcoded Rabies In Situ Connectomics for high-throughput reconstruction of neural circuits.Nature communications · 2026Article
- Large-scale mapping of the MCH network in ALS mice reveals the vulnerability of dopaminergic and GABAergic neurons in zona incerta.Acta neuropathologica communications · 2026Article
- Connectivity and phenotype of vasopressin 1a receptor cells in the lateral septum.bioRxiv : the preprint server for biology · 2025Article
- Circuit-specific gene editing for precision modulation of neuronal activity with CRISPR-rabies virus.bioRxiv : the preprint server for biology · 2025Article
- Transcriptomic Architecture of Weak Versus Strong Contextual Fear Extinction Learning Uncovers Extinction-Suppressive Role of TIA1.Biological psychiatry global open science · 2025Article
- Deconstruction of a spino-brain-spinal cord circuit drives chronic mechanical pain.Research square · 2025Article
- From thought to action: The organization of spinal projecting neurons.Cell reports · 2025Review
- The neural pathway from the superior subpart of the medial habenula to the interpeduncular nucleus suppresses anxiety.Molecular psychiatry · 2025Article
- A genetically defined pontine nucleus essential for ingestion in mice.Proceedings of the National Academy of Sciences of the United States of America · 2025Article
- Brain-wide connectivity and novelty response of the dorsal endopiriform nucleus in mice.Cell reports · 2025Article
- Thalamic CGRP neurons define a spinothalamic pathway for affective pain.Proceedings of the National Academy of Sciences of the United States of America · 2025Article
- Green-to-red spectral labeling: A novel polysynaptic retrograde tracing strategy in the marker footprint mouse model.Animal models and experimental medicine · 2025Article
- A food-sensitive olfactory circuit drives anticipatory satiety.Nature metabolism · 2025Article
- Neural connectivity of oxytocin receptor-expressing neurons in the nucleus accumbens and their role in social attachment.Hormones and behavior · 2025Article
- Article
- Customizable virus-like particles deliver CRISPR-Cas9 ribonucleoprotein for effective ocular neovascular and Huntington's disease gene therapy.Nature nanotechnology · 2025Article
- Adeno-associated viruses for efficient gene expression in the axolotl nervous system.Proceedings of the National Academy of Sciences of the United States of America · 2025Article
80 more citing papers are in PubMed but not listed here.
Corrections and comments
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Authors and funding
4 authors.
Funding
Abstract
Monosynaptic rabies virus tracing is a unique and powerful tool used to identify neurons making direct presynaptic connections onto neurons of interest across the entire nervous system. Current methods utilize complementation of glycoprotein gene-deleted rabies of the SAD B19 strain with its glycoprotein, B19G, to mediate retrograde transsynaptic spread across a single synaptic step. In most conditions, this method labels only a fraction of input neurons and would thus benefit from improved efficiency of transsynaptic spread. Here, we report newly engineered glycoprotein variants to improve transsynaptic efficiency. Among them, oG (optimized glycoprotein) is a codon-optimized version of a chimeric glycoprotein consisting of the transmembrane/cytoplasmic domain of B19G and the extracellular domain of rabies Pasteur virus strain glycoprotein. We demonstrate that oG increases the tracing efficiency for long-distance input neurons up to 20-fold compared to B19G. oG-mediated rabies tracing will therefore allow identification and study of more complete monosynaptic input neural networks.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.