ArticlePloS one2016
Nanopore-Based Target Sequence Detection.
Article in PloS one, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
17 citing papers in PubMed.
- Study on the controllability of the fabrication of single-crystal silicon nanopores/nanoslits with a fast-stop ionic current-monitored TSWE method.Microsystems & nanoengineering · 2023Article
- Perspectives on conformationally constrained peptide nucleic acid (PNA): insights into the structural design, properties and applications.RSC chemical biology · 2022Review
- Translocation Behaviors of Synthetic Polyelectrolytes through Alpha-Hemolysin (α-HL) andJournal of the Electrochemical Society · 2022Article
- Electronic Mapping of a Bacterial Genome with Dual Solid-State Nanopores and Active Single-Molecule Control.ACS nano · 2022Article
- Article
- Sequence-Specific Detection of DNA Strands Using a Solid-State Nanopore Assisted by Microbeads.Micromachines · 2020Article
- Sequence-specific detection of single-stranded DNA with a gold nanoparticle-protein nanopore approach.Scientific reports · 2020Article
- Solid-state nanopore fabrication by automated controlled breakdown.Nature protocols · 2020Article
- Stable fabrication of a large nanopore by controlled dielectric breakdown in a high-pH solution for the detection of various-sized molecules.Scientific reports · 2019Article
- Monolithic Fabrication of NPN/SiNAdvanced materials interfaces · 2019Article
- Synthesis of ( R)- and ( S)-Fmoc-Protected Diethylene Glycol Gamma PNA Monomers with High Optical Purity.The Journal of organic chemistry · 2019Article
- Assessment of eight nucleic acid amplification technologies for potential use to detect infectious agents in low-resource settings.PloS one · 2019Article
- Article
- A handheld platform for target protein detection and quantification using disposable nanopore strips.Scientific reports · 2018Article
- Two-step breakdown of a SiN membrane for nanopore fabrication: Formation of thin portion and penetration.Scientific reports · 2018Article
- DNA Translocations through Nanopores under Nanoscale Preconfinement.Nano letters · 2018Article
- Shape selective bifacial recognition of double helical DNA.Communications chemistry · 2018Article
Corrections and comments
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Authors and funding
8 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The promise of portable diagnostic devices relies on three basic requirements: comparable sensitivity to established platforms, inexpensive manufacturing and cost of operations, and the ability to survive rugged field conditions. Solid state nanopores can meet all these requirements, but to achieve high manufacturing yields at low costs, assays must be tolerant to fabrication imperfections and to nanopore enlargement during operation. This paper presents a model for molecular engineering techniques that meets these goals with the aim of detecting target sequences within DNA. In contrast to methods that require precise geometries, we demonstrate detection using a range of pore geometries. As a result, our assay model tolerates any pore-forming method and in-situ pore enlargement. Using peptide nucleic acid (PNA) probes modified for conjugation with synthetic bulk-adding molecules, pores ranging 15-50 nm in diameter are shown to detect individual PNA-bound DNA. Detection of the CFTRΔF508 gene mutation, a codon deletion responsible for ∼66% of all cystic fibrosis chromosomes, is demonstrated with a 26-36 nm pore size range by using a size-enhanced PNA probe. A mathematical framework for assessing the statistical significance of detection is also presented.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.