ArticlePloS one2016
Modulation of Osteogenesis in MC3T3-E1 Cells by Different Frequency Electrical Stimulation.
Article in PloS one, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 36 papers, 1 of them a synthesis that pooled it.
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Who cites it
36 citing papers in PubMed, 1 synthesis or guideline pooled it.
- Effect of electrical stimulation on the fusion rate after spinal surgery: a systematic review and meta-analysis.Neurosurgical review · 2024Pooled it
- In vitro electrical stimulation devices: practical framework for design, fabrication, and operation.Journal of biological engineering · 2026Article
- Enhanced Osteogenic and Neurogenic Differentiation of Dental Pulp Stem Cells by Micro-Current Stimulation and Chitosan-Poly(Ethylene Glycol) Blended Calcium Phosphate Cement.ACS applied bio materials · 2026Article
- Research on 3D-printed scaffolds with microstructure bio-inspired optimization for orbital bone defect repair.Journal of materials science. Materials in medicine · 2025Article
- Article
- Enhancing Bone Health with Conjugated Linoleic Acid: Mechanisms, Challenges, and Innovative Strategies.Nutrients · 2025Review
- Heterogeneous and Composite Bioinks for 3D-Bioprinting of Complex Tissue.Biomedical materials & devices (New York, N.Y.) · 2025Review
- Bone morphogenetic protein-2 and pulsed electrical stimulation synergistically promoted osteogenic differentiation on MC-3T3-E1 cells.Molecular and cellular biochemistry · 2024Article
- Recent Advancements in Bone Tissue Engineering: Integrating Smart Scaffold Technologies and Bio-Responsive Systems for Enhanced Regeneration.International journal of molecular sciences · 2024Review
- Direct coupled electrical stimulation towards improved osteogenic differentiation of human mesenchymal stem/stromal cells: a comparative study of different protocols.Scientific reports · 2024Article
- Electrical pulse stimulation parameters modulate N2a neuronal differentiation.Cell death discovery · 2024Article
- An Antibacterial, Conductive Nanocomposite Hydrogel Coupled with Electrical Stimulation for Accelerated Wound Healing.International journal of nanomedicine · 2024Article
- Effect of Electrical Stimulation on PC12 Cells Cultured in Different Hydrogels: Basis for the Development of Biomaterials in Peripheral Nerve Tissue Engineering.Pharmaceutics · 2023Article
- Discrimination between the effects of pulsed electrical stimulation and electrochemically conditioned medium on human osteoblasts.Journal of biological engineering · 2023Article
- Microenvironment-targeted strategy steers advanced bone regeneration.Materials today. Bio · 2023Review
- Electrical Stimulation of Human Adipose-Derived Mesenchymal Stem Cells on OInternational journal of molecular sciences · 2022Article
- DC electrical stimulation enhances proliferation and differentiation on N2a and MC3T3 cell lines.Journal of biological engineering · 2022Article
- New dual-function in situ bone repair scaffolds promote osteogenesis and reduce infection.Journal of biological engineering · 2022Article
- Interfaces Based on Laser-Structured Arrays of Carbon Nanotubes with Albumin for Electrical Stimulation of Heart Cell Growth.Polymers · 2022Article
- Integration of clinical perspective into biomimetic bioreactor design for orthopedics.Journal of biomedical materials research. Part B, Applied biomaterials · 2022Review
Corrections and comments
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Authors and funding
8 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Electrical stimulation (ES) is therapeutic to many bone diseases, from promoting fracture regeneration to orthopedic intervention. The application of ES offers substantial therapeutic potential, while optimal ES parameters and the underlying mechanisms responsible for the positive clinical impact are poorly understood. In this study, we assembled an ES cell culture and monitoring device. Mc-3T3-E1 cells were subjected to different frequency to investigate the effect of osteogenesis. Cell proliferation, DNA synthesis, the mRNA levels of osteosis-related genes, the activity of alkaline phosphatase (ALP), and intracellular concentration of Ca2+ were thoroughly evaluated. We found that 100 Hz could up-regulate the mRNA levels of collagen I, collagen II and Runx2. On the contrary, ES could down-regulate the mRNA levels of osteopontin (OPN). ALP activity assay and Fast Blue RR salt stain showed that 100 Hz could accelerate cells differentiation. Compared to the control group, 100 Hz could promote cell proliferation. Furthermore, 1 Hz to 10 Hz could improve calcium deposition in the intracellular matrix. Overall, these results indicate that 100Hz ES exhibits superior potentialities in osteogenesis, which should be beneficial for the clinical applications of ES for the treatment of bone diseases.
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Registered trials
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