ArticlePLoS genetics2016
Germline Defects Caused by Smed-boule RNA-Interference Reveal That Egg Capsule Deposition Occurs Independently of Fertilization, Ovulation, Mating, or the Presence of Gametes in Planarian Flatworms.
Article in PLoS genetics, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
14 citing papers in PubMed, 17 citations in OpenAlex.
- Drift of positional identity drives reproductive aging in a long-lived regenerative animal.Current biology : CB · 2026Article
- Conserved innate immunity components limit transgene expression in adult planarians.Development (Cambridge, England) · 2026Article
- A Tousled-like kinase (TLK) homolog required for male germline stem cell homeostasis in the planarian Schmidtea mediterranea.Developmental biology · 2026Article
- Melastatin family Transient Receptor Potential channels support spermatogenesis in planarian flatworms.The International journal of developmental biology · 2025Article
- Article
- Whole-Mount In Situ Hybridization in Large Sexual Schmidtea mediterranea.Methods in molecular biology (Clifton, N.J.) · 2023Article
- Analysis of Morphogenesis and Flagellar Assembly During Spermatogenesis in Planarian Flatworms.Methods in molecular biology (Clifton, N.J.) · 2022Article
- Single-cell analysis of Schistosoma mansoni identifies a conserved genetic program controlling germline stem cell fate.Nature communications · 2021Article
- Tau tubulin kinase is required for spermatogenesis and development of motile cilia in planarian flatworms.Molecular biology of the cell · 2019Article
- Insights into the histology of planarian flatworm Phagocata gracilis based on location specific, intact lipid information provided by GCIB-ToF-SIMS imaging.Biochimica et biophysica acta. Molecular and cell biology of lipids · 2019Article
- Genome-wide transcriptome profiling and spatial expression analyses identify signals and switches of development in tapeworms.EvoDevo · 2018Article
- Control of Western Corn Rootworm (Diabrotica virgifera virgifera) Reproduction through Plant-Mediated RNA Interference.Scientific reports · 2017Article
- Genetic dissection of the planarian reproductive system through characterization of Schmidtea mediterranea CPEB homologs.Developmental biology · 2017Article
- Article
Corrections and comments
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
Abstract
Few animals are known to lay eggs in the absence of ovulation or copulation, as it is presumably energetically wasteful and subjected to negative selection. Characterization of Smed-boule, a member of the DAZ family of germline RNA-binding proteins, revealed that egg capsule (or capsule) production and deposition occurs independently of the presence of gametes in the planarian flatworm Schmidtea mediterranea. Reduction of Smed-boule expression by RNA-interference (RNAi) causes ablation of spermatogonial stem cells and the inability of ovarian germline stem cells to undergo oogenesis. Although animals subjected to Smed-boule RNAi lose their gametes and become sterile, they continue to lay egg capsules. Production of sterile capsules is even observed in virgin Smed-boule(RNAi) and control planarians maintained in complete isolation, demonstrating that egg production in S. mediterranea occurs independently of ovulation, fertilization, or mating. Evidence suggests that this is a conserved feature amongst Platyhelminthes, and therefore relevant to the pathology and dissemination of parasitic flatworms. These findings demonstrate that Smed-boule functions at different stages during male and female germline stem cell development, and also demonstrate that egg capsule production by planarian flatworms occurs independently of signals produced by mating or ova.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.