ArticlePloS one2016
Comparing the Effects of Isoflurane and Alpha Chloralose upon Mouse Physiology.
Article in PloS one, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 28 papers, 1 of them a synthesis that pooled it.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
28 citing papers in PubMed, 1 synthesis or guideline pooled it.
- Preclinical techniques to investigate exercise training in vascular pathophysiology.American journal of physiology. Heart and circulatory physiology · 2021Pooled it
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- The non-invasive transcranial Doppler for hemodynamic monitoring.Physiological research · 2025Article
- Reactivated thalamocortical plasticity alters neural activity in sensory-motor cortex during post-critical period.Progress in neurobiology · 2025Article
- High-resolution awake mouse fMRI at 14 tesla.eLife · 2025Article
- Cardiovascular function and autonomic regulation in urethane-anesthetized and conscious mice.American journal of physiology. Regulatory, integrative and comparative physiology · 2025Article
- Enhancing anesthetic techniques for improving whisker stimulation response in the barrel cortex.PloS one · 2025Article
- High-resolution awake mouse fMRI at 14 Tesla.bioRxiv : the preprint server for biology · 2024Article
- Existence of multiple transitions of the critical state due to anesthetics.Nature communications · 2024Article
- Jiaohong pills attenuate neuroinflammation and amyloid-β protein-induced cognitive deficits by modulating the mitogen-activated protein kinase/nuclear factor kappa-B pathway.Animal models and experimental medicine · 2024Article
- Central Artery Hemodynamics in Angiotensin II-Induced Hypertension and Effects of Anesthesia.Annals of biomedical engineering · 2024Article
- Oxygen imaging of hypoxic pockets in the mouse cerebral cortex.Science (New York, N.Y.) · 2024Article
- RUNX1-PDIA5 Axis Promotes Malignant Progression of Glioblastoma by Regulating CCAR1 Protein Expression.International journal of biological sciences · 2024Article
- Opioid Overdose Detection in a Murine Model Using a Custom-Designed Photoplethysmography Device.Ingenierie et recherche biomedicale : IRBM = Biomedical engineering and research · 2023Article
- Simultaneous magnetic resonance imaging of pH, perfusion and renal filtration using hyperpolarizedNature communications · 2023Article
- Intrinsic, widefield optical imaging of hemodynamics in rodent models of Alzheimer's disease and neurological injury.Neurophotonics · 2023Review
- Article
- Anesthetic modulations dissociate neuroelectric characteristics between sensory-evoked and spontaneous activities across bilateral rat somatosensory cortical laminae.Scientific reports · 2022Article
- Protective effect of isoflurane preconditioning on neurological function in rats with HIE.Ibrain · 2022Article
- Mouse Anesthesia: The Art and Science.ILAR journal · 2021Review
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
3 authors.
Funding
Abstract
Functional magnetic resonance imaging of mice requires that the physiology of the mouse (body temperature, respiration and heart rates, blood pH level) be maintained in order to prevent changes affecting the outcomes of functional scanning, namely blood oxygenation level dependent (BOLD) measures and cerebral blood flow (CBF). The anesthetic used to sedate mice for scanning can have major effects on physiology. While alpha chloralose has been commonly used for functional imaging of rats, its effects on physiology are not well characterized in the literature for any species. In this study, we anesthetized or sedated mice with isoflurane or alpha chloralose for up to two hours, and monitored physiological parameters and arterial blood gasses. We found that, when normal body temperature is maintained, breathing rates for both drugs decrease over the course of two hours. In addition, alpha chloralose causes a substantial drop in heart rate and blood pH with severe hypercapnia (elevated blood CO2) that is not seen in isoflurane-treated animals. We suggest that alpha chloralose does not maintain normal mouse physiology adequately for functional brain imaging outcome measures.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.