ArticleJournal of clinical virology : the official publication of the Pan American Society for Clinical Virology2016
Validation of a standardized extraction method for formalin-fixed paraffin-embedded tissue samples.
Article in Journal of clinical virology : the official publication of the Pan American Society for Clinical Virology, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
17 citing papers in PubMed, 30 citations in OpenAlex.
- Human papillomavirus self-sampling with mRNA testing benefits routine screening.International journal of cancer · 2022Trial
- Human papillomavirus vaccine efficacy against invasive, HPV-positive cancers: population-based follow-up of a cluster-randomised trial.BMJ open · 2021Trial
- Trial
- Human papillomavirus detection in liquid-based cytology and cervical histology samples among women living with human immunodeficiency virus in Zambia.IJID regions · 2026Article
- Cervical cancer microbiome analysis: comparing HPV 16 and 18 with other HPV types.Scientific reports · 2024Article
- Human papillomavirus negative high grade cervical lesions and cancers: Suggested guidance for HPV testing quality assurance.Journal of clinical virology : the official publication of the Pan American Society for Clinical Virology · 2024Article
- Evaluation of the Novaplex II HPV28 Detection Assay for HPV Typing in Formalin-Fixed, Paraffin-Embedded Tissues.The Journal of molecular diagnostics : JMD · 2023Article
- Article
- Article
- Technical challenges regarding the use of formalin-fixed paraffin embedded (FFPE) tissue specimens for the detection of bacterial alterations in colorectal cancer.BMC microbiology · 2021Article
- Differing Age-Specific Cervical Cancer Incidence Between Different Types of Human Papillomavirus: Implications for Predicting the Impact of Elimination Programs.American journal of epidemiology · 2021Article
- A fluorometric hybridization assay for detecting and genotyping high-risk human papillomavirus 16 and 18 in archival tissues of cervical specimens.Brazilian journal of microbiology : [publication of the Brazilian Society for Microbiology] · 2020Article
- Cervical screening and risk of adenosquamous and rare histological types of invasive cervical carcinoma: population based nested case-control study.BMJ (Clinical research ed.) · 2019Article
- Human papillomavirus types in cervical high-grade lesions or cancer among Nordic women-Potential for prevention.Cancer medicine · 2019Article
- High-risk human papillomavirus status and prognosis in invasive cervical cancer: A nationwide cohort study.PLoS medicine · 2018Article
- Nationwide comprehensive human papillomavirus (HPV) genotyping of invasive cervical cancer.British journal of cancer · 2018Article
- Mitochondrial DNA ancestry, HPV infection and the risk of cervical cancer in a multiethnic population of northeastern Argentina.PloS one · 2018Article
Corrections and comments
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Authors and funding
6 authors at 2 institutions in 2 countries.
Funding
Abstract
backgroundFormalin-fixed paraffin-embedded (FFPE) samples can be DNA-extracted and used for human papillomavirus (HPV) genotyping. The xylene-based gold standard for extracting FFPE samples is laborious, suboptimal and involves health hazards for the personnel involved.
objectivesTo compare extraction with the standard xylene method to a xylene-free method used in an HPV LabNet Global Reference Laboratory at the Centers for Disease Control (CDC); based on a commercial method with an extra heating step. STUDY
designFifty FFPE samples were randomly selected from a national audit of all cervical cancer cases diagnosed in Sweden during 10 years. For each case-block, a blank-block was sectioned, as a control for contamination. For xylene extraction, the standard WHO Laboratory Manual protocol was used. For the CDC method, the manufacturers' protocol was followed except for an extra heating step, 120°C for 20min. Samples were extracted and tested in parallel with β-globin real-time PCR, HPV16 real-time PCR and HPV typing using modified general primers (MGP)-PCR and Luminex assays.
resultsFor a valid result the blank-block had to be betaglobin-negative in all tests and the case-block positive for beta-globin. Overall, detection was improved with the heating method and the amount of HPV-positive samples increased from 70% to 86% (p=0.039). For all samples where HPV type concordance could be evaluated, there was 100% type concordance.
conclusionsA xylene-free and robust extraction method for HPV-DNA typing in FFPE material is currently in great demand. Our proposed standardized protocol appears to be generally useful.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.