ReviewFrontiers in genetics2016
Gene Targeting Using Homologous Recombination in Embryonic Stem Cells: The Future for Behavior Genetics?
Review in Frontiers in genetics, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 12 papers, 1 of them a synthesis that pooled it.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
12 citing papers in PubMed, 1 synthesis or guideline pooled it.
- Pooled it
- Recent Advances in Antibiotic-Free Markers; Novel Technologies to Enhance Safe Human Food Production in the World.Molecular biotechnology · 2023Review
- Rodent genetic models of Ah receptor signaling.Drug metabolism reviews · 2021Review
- In vivo enrichment of busulfan-resistant germ cells for efficient production of transgenic avian models.Scientific reports · 2021Article
- CRISPR Toolbox for Genome Editing inFrontiers in cell and developmental biology · 2021Article
- Targeted Gene Knockouts by Protoplast Transformation in the MossFrontiers in genome editing · 2021Review
- Adaptation of the AID system for stem cell and transgenic mouse research.Stem cell research · 2020Article
- Stress in groups: Lessons from non-traditional rodent species and housing models.Neuroscience and biobehavioral reviews · 2020Review
- Dystrophin and calcium current are decreased in cardiomyocytes expressing Cre enzyme driven by αMHC but not TNT promoter.Scientific reports · 2019Article
- Streamlined computational pipeline for genetic background characterization of genetically engineered mice based on next generation sequencing data.BMC genomics · 2019Article
- Comprehensive behavioral analysis of indoleamine 2,3-dioxygenase knockout mice.Neuropsychopharmacology reports · 2018Article
- Efficient transgenesis and annotated genome sequence of the regenerative flatworm model Macrostomum lignano.Nature communications · 2017Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
1 author.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Gene targeting with homologous recombination in embryonic stem cells created a revolution in the analysis of the function of genes in behavioral brain research. The technology allowed unprecedented precision with which one could manipulate genes and study the effect of this manipulation on the central nervous system. With gene targeting, the uncertainty inherent in psychopharmacology regarding whether a particular compound would act only through a specific target was removed. Thus, gene targeting became highly popular. However, with this popularity came the realization that like other methods, gene targeting also suffered from some technical and principal problems. For example, two decades ago, issues about compensatory changes and about genetic linkage were raised. Since then, the technology developed, and its utility has been better delineated. This review will discuss the pros and cons of the technique along with these advancements from the perspective of the neuroscientist user. It will also compare and contrast methods that may represent novel alternatives to the homologous recombination based gene targeting approach, including the TALEN and the CRISPR/Cas9 systems. The goal of the review is not to provide detailed recipes, but to attempt to present a short summary of these approaches a behavioral geneticist or neuroscientist may consider for the analysis of brain function and behavior.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.