ArticleFrontiers in microbiology2016
Identification of the Genes Required for the Culture of Liberibacter crescens, the Closest Cultured Relative of the Liberibacter Plant Pathogens.
Article in Frontiers in microbiology, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 27 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
27 citing papers in PubMed, 43 citations in OpenAlex.
- A secreted citrus protease cleaves an outer membrane protein of the Huanglongbing pathogen.Proceedings of the National Academy of Sciences of the United States of America · 2026Article
- Galleria mellonella possesses the essential nutritional needs to host the fastidious Huanglongbing bacterial pathogen 'Candidatus Liberibacter asiaticus'.Communications biology · 2025Article
- Identification and functional analysis of putative effector proteins fromFrontiers in plant science · 2025Article
- Transcriptomic analysis of early stages of 'Frontiers in plant science · 2025Article
- The protein interactome of the citrus Huanglongbing pathogen Candidatus Liberibacter asiaticus.Nature communications · 2023Article
- A protease and a lipoprotein jointly modulate the conserved ExoR-ExoS-ChvI signaling pathway critical in Sinorhizobium meliloti for symbiosis with legume hosts.PLoS genetics · 2023Article
- A synthetic 'essentialome' for axenic culturing of 'Candidatus Liberibacter asiaticus'.BMC research notes · 2022Article
- Proteomic and bioinformatic analyses of proteins in the outer membrane and extracellular compartments and outer membrane vesicles ofFrontiers in microbiology · 2022Article
- Comparative Phylogenomic Analysis Reveals Evolutionary Genomic Changes and Novel Toxin Families in EndophyticMicrobiology spectrum · 2021Article
- Linking metabolic phenotypes to pathogenic traits among "Candidatus Liberibacter asiaticus" and its hosts.NPJ systems biology and applications · 2020Article
- Zinkicide Is a ZnO-Based Nanoformulation with Bactericidal Activity against Liberibacter crescens in Batch Cultures and in Microfluidic Chambers Simulating Plant Vascular Systems.Applied and environmental microbiology · 2020Article
- Genome-wide analyses of Liberibacter species provides insights into evolution, phylogenetic relationships, and virulence factors.Molecular plant pathology · 2020Article
- Application of Transposon Insertion Sequencing to Agricultural Science.Frontiers in plant science · 2020Review
- Transcriptomic and Metabolomic Analyses ofFrontiers in physiology · 2020Article
- Host-free biofilm culture of "Biofilm · 2019Article
- Physiochemical changes mediated by "Candidatus Liberibacter asiaticus" in Asian citrus psyllids.Scientific reports · 2019Article
- Growth parameters of Liberibacter crescens suggest ammonium and phosphate as essential molecules in the Liberibacter-plant host interface.BMC microbiology · 2019Article
- Review
- A high-throughput system to identify inhibitors ofProceedings of the National Academy of Sciences of the United States of America · 2019Article
- Liberibacter crescens biofilm formation in vitro: establishment of a model system for pathogenic 'Candidatus Liberibacter spp.'Scientific reports · 2019Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
4 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Here Tn5 random transposon mutagenesis was used to identify the essential elements for culturing Liberibacter crescens BT-1 that can serve as antimicrobial targets for the closely related pathogens of citrus, Candidatus Liberibacter asiaticus (Las) and tomato and potato, Candidatus Liberibacter solanacearum (Lso). In order to gain insight on the virulence, metabolism, and culturability of the pathogens within the genus Liberibacter, a mini-Tn5 transposon derivative system consisting of a gene specifying resistance to kanamycin, flanked by a 19-base-pair terminal repeat sequence of Tn5, was used for the genome-wide mutagenesis of L. crescens BT-1 and created an insertion mutant library. By analyzing the location of insertions using Sanger and Illumina Mi-Seq sequencing, 314 genes are proposed as essential for the culture of L. crescens BT-1 on BM-7 medium. Of those genes, 76 are not present in the uncultured Liberibacter pathogens and, as a result, suggest molecules necessary for the culturing these pathogens. Those molecules include the aromatic amino acids, several vitamins, histidine, cysteine, lipopolysaccharides, and fatty acids. In addition, the 238 essential genes of L. crescens in common with L. asiaticus are potential targets for the development of therapeutics against the disease.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.