Evidence map›Paper›PMID 27147050›Full record

ArticleMethods in molecular biology (Clifton, N.J.)2016

Imaging Approaches to Investigate Myonuclear Positioning in Drosophila.

Mafalda Azevedo, Victoria K Schulman, Eric Folker, Mridula Balakrishnan, Mary Baylies

Abstract read
In one paragraph

Article in Methods in molecular biology (Clifton, N.J.), 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed.

  1. Article
  2. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

5 authors.

Mafalda AzevedoGraduate Program in Basic and Applied Biology (GABBA), Institute of Biomedical Sciences Abel Salazar, University of Porto, Porto, Portugal.
Victoria K SchulmanProgram in Developmental Biology, Sloan Kettering Institute, Baylies Lab, Box 310, 1275 York Avenue, New York, NY, 10065, USA.
Eric FolkerProgram in Developmental Biology, Sloan Kettering Institute, Baylies Lab, Box 310, 1275 York Avenue, New York, NY, 10065, USA.
Mridula BalakrishnanProgram in Developmental Biology, Sloan Kettering Institute, Baylies Lab, Box 310, 1275 York Avenue, New York, NY, 10065, USA.
Mary BayliesProgram in Developmental Biology, Sloan Kettering Institute, Baylies Lab, Box 310, 1275 York Avenue, New York, NY, 10065, USA. m-baylies@ski.mskcc.org.

Funding

X-RAY CRYSTALLOGRAPHYP30CA008748 · NCI · SLOAN-KETTERING INSTITUTE FOR CANCER RES · PI Nicholas D Socci · 1985 to 2026
$347.4M
Mechanisms and Function of Myonuclear PositioningR01AR068128 · NIAMS · SLOAN-KETTERING INST CAN RESEARCH · PI BAYLIES, MARY K · 2014 to 2025
$5.1M
Therapeutic target discovery in Drosophila models of Nemaline MyopathyR21AR067361 · NIAMS · SLOAN-KETTERING INST CAN RESEARCH · PI BAYLIES, MARY K · 2015 to 2016
$426k
NCI NIH HHS P30 CA008748NIAMS NIH HHS R01 AR068128NIAMS NIH HHS R21 AR067361
6 · The paper itself

Abstract

In the skeletal muscle, nuclei are positioned at the periphery of each myofiber and are evenly distributed along its length. Improper positioning of myonuclei has been correlated with muscle disease and decreased muscle function. Several mechanisms required for regulating nuclear position have been identified using the fruit fly, Drosophila melanogaster. The conservation of the myofiber between the fly and vertebrates, the availability of advanced genetic tools, and the ability to visualize dynamic processes using fluorescent proteins in vivo makes the fly an excellent system to study myonuclear positioning. This chapter describes time-lapse and fixed imaging methodologies using both the Drosophila embryo and the larva to investigate mechanisms of myonuclear positioning.

Indexed as

Microscopy, FluorescenceTime-Lapse ImagingAnimalsCell NucleusDrosophilaEmbryo, NonmammalianImage Processing, Computer-AssistedMuscle Fibers, SkeletalMuscle, SkeletalDrosophilaEmbryoLarvaeMuscleNuclear movement

Identifiers

PMID27147050
PMCPMC4939885

What OpenQuestion holds

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Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.