Probing Protein Distribution Along the Nuclear Envelope In Vivo by Using Single-Point FRAP.

Krishna C Mudumbi, Weidong Yang

Abstract read

In one paragraph

Article in Methods in molecular biology (Clifton, N.J.), 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it

0cells of the map it votes in

0citing papers in PubMed

–field-weighted citation impact

1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

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Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

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0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

2 authors.

Krishna C MudumbiDepartment of Biology, Temple University, 1900 North 12th St., Philadelphia, PA, 19122, USA.

Weidong YangDepartment of Biology, Temple University, 1900 North 12th St., Philadelphia, PA, 19122, USA. weidong.yang@temple.edu.

Funding

Super-resolution Microscopy Study of Molecular Transport MechanismsR35GM122552 · NIGMS · TEMPLE UNIV OF THE COMMONWEALTH · PI Weidong Yang · 2017 to 2026

$4.5M

Structure and Function of the Ciliary Pore ComplexR01GM116204 · NIGMS · UNIVERSITY OF MICHIGAN AT ANN ARBOR · PI VERHEY, KRISTEN J., YANG, WEIDONG · 2015 to 2018

$2.1M

Three-Dimensional Super-Resolution Microscopy Study of Nuclear TransportR01GM097037 · NIGMS · TEMPLE UNIV OF THE COMMONWEALTH · PI YANG, WEIDONG · 2011 to 2015

$1.3M

NIGMS NIH HHS R01 GM097037NIGMS NIH HHS R01 GM116204NIGMS NIH HHS R35 GM122552

6 · The paper itself

Abstract

Determining the locations of nuclear envelope transmembrane proteins and their concentrations across the outer and inner nuclear membranes has been a challenging and time-consuming process. Typically, this required the week-long process of fixing and immunogold staining of cells prior to analysis by electron microscopy. Here, we describe a method, single-point fluorescence recovery after photobleaching (spFRAP), which is able to quickly determine the localization and distribution of nuclear membrane proteins along the double nuclear envelope membranes with a precision of 10-15 nm in a matter of 10-20 min the day after transfection.

Indexed as

Fluorescence Recovery After PhotobleachingGene ExpressionGenes, ReporterHeLa CellsHumansMembrane ProteinsNuclear EnvelopeProtein TransportTransfectionMembrane ProteinsFluorescence recovery after photobleachingNuclear envelope transmembrane proteinsSingle moleculeSuper-resolution

Identifiers

PMID27147037

DOI10.1007/978-1-4939-3530-7_6

PMCPMC10099394

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.