Evidence map›Paper›PMID 27146879›Full record

ArticleEuropean journal of clinical microbiology & infectious diseases : official publication of the European Society of Clinical Microbiology2016

Direct molecular typing of Bordetella pertussis from nasopharyngeal specimens in China in 2012-2013.

Q Du, X Wang, Y Liu, Y Luan, J Zhang, Y Li, X Liu, C Ma, H Li, Z Wang and 1 more

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Article in European journal of clinical microbiology & infectious diseases : official publication of the European Society of Clinical Microbiology, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 12 papers.

0numbers the graph read from it
0cells of the map it votes in
12citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

12 citing papers in PubMed.

  1. Vaccine antigen-based genotyping ofMicrobiology spectrum · 2025
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  2. Article
  3. Article
  4. Article
  5. Genotypes ofZhejiang da xue xue bao. Yi xue ban = Journal of Zhejiang University. Medical sciences · 2022
    Article
  6. Article
  7. Article
  8. Population dynamics and antigenic drift ofEmerging microbes & infections · 2019
    Article
  9. Article
  10. Article
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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

11 authors.

Q DuXi'an Center for Disease Control and Prevention, Xi'an, China.
X WangXi'an Center for Disease Control and Prevention, Xi'an, China.
Y LiuXi'an Center for Disease Control and Prevention, Xi'an, China.
Y LuanXi'an Center for Disease Control and Prevention, Xi'an, China.
J ZhangXi'an Center for Disease Control and Prevention, Xi'an, China.
Y LiDepartment of Infectious Diseases, Xi'an Children Hospital, Xi'an, China.
X LiuDepartment of Infectious Diseases, Xi'an Children Hospital, Xi'an, China.
C MaXi'an Center for Disease Control and Prevention, Xi'an, China.
H LiXi'an Center for Disease Control and Prevention, Xi'an, China.
Z WangXi'an Center for Disease Control and Prevention, Xi'an, China. wangzengguo@xiancdc.cn.
Q HeDepartment of Medical Microbiology, Capital Medical University, Beijing, China. Qiushui.He@utu.fi.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Data on the molecular epidemiology of Bordetella pertussis are limited in developing countries where whole-cell pertussis vaccines (WCVs) have been used. The aim of this study was to determine the genotypes of circulating B. pertussis in China by direct molecular typing of clinical specimens. DNA extracts of 122 nasopharyngeal swabs (NPs) positive for B. pertussis by polymerase chain reaction (PCR) (targeting IS481 and ptx-Pr) from 2012 to 2013 were used for typing using the multiple-locus variable number tandem repeat analysis (MLVA) and also by PCR-based multilocus sequence typing (MLST) of B. pertussis virulence genes (ptxP, prn, and fim3). One hundred and eight DNA extracts (89 %) generated a complete MLVA type (MT). Among the 18 MTs obtained, MT55 (52 %) and MT104 (13 %) were the most common. MT27, which is linked to the ptxP3 allele and is prevalent in many developed countries using acellular pertussis vaccines (ACVs), was only found in 7 (6 %) DNA extracts. Eighty-seven DNA extracts (71 %) produced a complete multiantigen sequence typing (MAST) type. Of them, 77 (89 %) had the ptxP1/prn1/fim3-1 allele profile. Four DNA extracts (5 %) had the ptxP3/prn2/fim3-2 profile and 3 (4 %) had the ptxP3/prn1/fim3-2 allele profile. These seven DNA extracts also harbored MT27. Our result shows that B. pertussis circulating in China was different from those found in countries where ACVs have been in use, supporting the notion that selection pressure induced by WCVs and ACVs on the bacterial population differs.

Indexed as

Bordetella pertussisChinaCluster AnalysisGeographyHistory, 21st CenturyHumansMolecular TypingMultilocus Sequence TypingNasopharynxWhooping Cough

Identifiers

PMID27146879

What OpenQuestion holds

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.