ArticleScientific reports2016
Rapid authentication of the precious herb saffron by loop-mediated isothermal amplification (LAMP) based on internal transcribed spacer 2 (ITS2) sequence.
Article in Scientific reports, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
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Who cites it
18 citing papers in PubMed.
- Loop-mediated isothermal amplification (LAMP) for quality control of Coscinium fenestratum: a colorimetric platform for detecting Anamirta cocculus adulteration.Protoplasma · 2026Article
- Shedding light on deception: LAMP as a tool for detecting adulterants in herbal medicine.Protoplasma · 2026Review
- Epigenetic-modifications induced by plant-microbial interactions modulate plant immunity, Defense-response and mutualistic associations.Molecular biology reports · 2025Review
- On site discrimination between two closely related commercial strains of oyster mushroom using a loop-mediated isothermal amplification (LAMP) test.Molecular biology reports · 2025Article
- Single nucleotide polymorphism-based visual identification ofFrontiers in plant science · 2024Article
- Genetic Authentication of the Medicinal PlantInternational journal of molecular sciences · 2023Article
- Phytochemistry, quality control and medicinal uses of Saffron (Journal of medicine and life · 2023Review
- DNA barcoding in herbal medicine: Retrospective and prospective.Journal of pharmaceutical analysis · 2023Review
- Horizon scan of DNA-based methods for quality control and monitoring of herbal preparations.Frontiers in pharmacology · 2023Review
- TaqMan Probe-Based Quantitative Real-Time PCR to DetectFrontiers in pharmacology · 2022Article
- Advancements and future prospective of DNA barcodes in the herbal drug industry.Frontiers in pharmacology · 2022Review
- Specific, sensitive and rapidSaudi journal of biological sciences · 2021Article
- Molecular Approaches to Agri-Food Traceability and Authentication: An Updated Review.Foods (Basel, Switzerland) · 2021Review
- A simple and effective method to discern the true commercial Chinese cordyceps from counterfeits.Scientific reports · 2020Article
- Specific and rapid identification of theBioscience reports · 2019Article
- Using SSR-HRM to Identify Closely Related Species in Herbal Medicine Products: A Case Study on Licorice.Frontiers in pharmacology · 2018Article
- A Comprehensive Quality Evaluation System for Complex Herbal Medicine Using PacBio Sequencing, PCR-Denaturing Gradient Gel Electrophoresis, and Several Chemical Approaches.Frontiers in plant science · 2017Article
- Loop-Mediated Isothermal Amplification (LAMP): Emergence As an Alternative Technology for Herbal Medicine Identification.Frontiers in plant science · 2016Review
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9 authors.
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No grant is acknowledged in the PubMed record.
Abstract
Saffron is one of the most expensive species of Chinese herbs and has been subjected to various types of adulteration because of its high price and limited production. The present study introduces a loop-mediated isothermal amplification (LAMP) technique for the differentiation of saffron from its adulterants. This novel technique is sensitive, efficient and simple. Six specific LAMP primers were designed on the basis of the nucleotide sequence of the internal transcribed spacer 2 (ITS2) nuclear ribosomal DNA of Crocus sativus. All LAMP amplifications were performed successfully, and visual detection occurred within 60 min at isothermal conditions of 65 °C. The results indicated that the LAMP primers are accurate and highly specific for the discrimination of saffron from its adulterants. In particular, 10 fg of genomic DNA was determined to be the limit for template accuracy of LAMP in saffron. Thus, the proposed novel, simple, and sensitive LAMP assay is well suited for immediate on-site discrimination of herbal materials. Based on the study, a practical standard operating procedure (SOP) for utilizing the LAMP protocol for herbal authentication is provided.
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