ArticleMolecular biology of the cell2016
GDP-to-GTP exchange on the microtubule end can contribute to the frequency of catastrophe.
Article in Molecular biology of the cell, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 29 papers.
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Who cites it
29 citing papers in PubMed, 61 citations in OpenAlex.
- Many will enter, few will win: Cost and sensitivity of exploratory dynamics.Biophysical journal · 2026Article
- Single-molecule microtubule dynamics measurements reveal an intermediate state and clarify the role of nucleotide.Nature communications · 2025Article
- Mechanisms of microtubule dynamics from single-molecule measurements.bioRxiv : the preprint server for biology · 2025Article
- Modulating the Curvature of Protein Self-Assembled Spiral Nanotubules.ACS applied materials & interfaces · 2025Article
- Distinct Network Morphologies from In Situ Polymerization of Microtubules in Giant Polymer-Lipid Hybrid Vesicles.Advanced biology · 2025Article
- EB3-informed dynamics of the microtubule stabilizing cap during stalled growth.Biophysical journal · 2025Article
- Interface-acting nucleotide controls polymerization dynamics at microtubule plus- and minus-ends.eLife · 2024Article
- Unveiling the catalytic mechanism of GTP hydrolysis in microtubules.Proceedings of the National Academy of Sciences of the United States of America · 2023Article
- CLASPs stabilize the pre-catastrophe intermediate state between microtubule growth and shrinkage.The Journal of cell biology · 2023Article
- CLASP2 recognizes tubulins exposed at the microtubule plus-end in a nucleotide state-sensitive manner.Science advances · 2023Article
- Beyond the GTP-cap: Elucidating the molecular mechanisms of microtubule catastrophe.BioEssays : news and reviews in molecular, cellular and developmental biology · 2023Article
- Article
- Reconstituting Microtubules: A Decades-Long Effort From Building Block Identification to the Generation of Recombinant α/β-Tubulin.Frontiers in cell and developmental biology · 2022Review
- Programmed mechano-chemical coupling in reaction-diffusion active matter.Science advances · 2021Article
- XMAP215 promotes microtubule catastrophe by disrupting the growing microtubule end.The Journal of cell biology · 2021Article
- Atomistic Basis of Microtubule Dynamic Instability Assessed Via Multiscale Modeling.Annals of biomedical engineering · 2021Article
- Molecular mechanisms underlying microtubule growth dynamics.Current biology : CB · 2021Review
- Microtubules form by progressively faster tubulin accretion, not by nucleation-elongation.The Journal of cell biology · 2021Article
- Microtubule Dysfunction: A Common Feature of Neurodegenerative Diseases.International journal of molecular sciences · 2020Review
- Tubulin islands containing slowly hydrolyzable GTP analogs regulate the mechanism and kinetics of microtubule depolymerization.Scientific reports · 2020Article
Corrections and comments
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Authors and funding
7 authors at 1 institution in 1 country.
Funding
Abstract
Microtubules are dynamic polymers of αβ-tubulin that have essential roles in chromosome segregation and organization of the cytoplasm. Catastrophe-the switch from growing to shrinking-occurs when a microtubule loses its stabilizing GTP cap. Recent evidence indicates that the nucleotide on the microtubule end controls how tightly an incoming subunit will be bound (trans-acting GTP), but most current models do not incorporate this information. We implemented trans-acting GTP into a computational model for microtubule dynamics. In simulations, growing microtubules often exposed terminal GDP-bound subunits without undergoing catastrophe. Transient GDP exposure on the growing plus end slowed elongation by reducing the number of favorable binding sites on the microtubule end. Slower elongation led to erosion of the GTP cap and an increase in the frequency of catastrophe. Allowing GDP-to-GTP exchange on terminal subunits in simulations mitigated these effects. Using mutant αβ-tubulin or modified GTP, we showed experimentally that a more readily exchangeable nucleotide led to less frequent catastrophe. Current models for microtubule dynamics do not account for GDP-to-GTP exchange on the growing microtubule end, so our findings provide a new way of thinking about the molecular events that initiate catastrophe.
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