ArticleMolecular microbiology2016
A scaffold protein connects type IV pili with the Chp chemosensory system to mediate activation of virulence signaling in Pseudomonas aeruginosa.
Article in Molecular microbiology, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 63 papers.
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Who cites it
63 citing papers in PubMed, 94 citations in OpenAlex.
- Bacteria regulate collective behaviour by mechanosensing cell-cell collisions.Nature microbiology · 2026Article
- Distinct roles of PilI and ChpC inApplied and environmental microbiology · 2026Article
- Injectisome assembly primesmBio · 2026Article
- A farnesol-sensing triad inProceedings of the National Academy of Sciences of the United States of America · 2026Article
- PilY proteins: bimodular drivers of type IV pilus versatility.Trends in microbiology · 2026Review
- Functional characterization of the polar organizer protein FimV inJournal of bacteriology · 2026Article
- Transcriptional profiling ofFrontiers in microbiology · 2026Article
- The type IV pilus steering committee: how Pil-Chp controls directional motility.Journal of bacteriology · 2025Review
- Separation ofmBio · 2025Article
- Twitching motility suppressors reveal a role for FimX in type IV pilus extension dynamics.PLoS genetics · 2025Article
- FimX regulates type IV pilus localization via the Pil-Chp chemosensory system inMolecular biology of the cell · 2025Article
- Genetic analysis of flagellar-mediated surface sensing byJournal of bacteriology · 2025Article
- Antagonistic response regulators spatially regulate receptor methylation in the Pseudomonas aeruginosa Pil-Chp surface sensing system.Cell reports · 2025Article
- Recent advances in high-throughput screening methods for small molecule modulators in bacteria.Current opinion in chemical biology · 2025Review
- Bloodstream infections: mechanisms of pathogenesis and opportunities for intervention.Nature reviews. Microbiology · 2025Review
- Physical communication pathways in bacteria: an extra layer to quorum sensing.Biophysical reviews · 2025Review
- Structural conservation and functional role of TfpY-like proteins in type IV pilus assembly.Journal of bacteriology · 2025Article
- Building permits-control of type IV pilus assembly by PilB and its cofactors.Journal of bacteriology · 2024Review
- Article
- ThemSphere · 2024Article
3 more citing papers are in PubMed but not listed here.
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Authors and funding
8 authors at 3 institutions in 1 country.
Funding
Abstract
Type IV pili (TFP) function as mechanosensors to trigger acute virulence programs in Pseudomonas aeruginosa. On surface contact, TFP retraction activates the Chp chemosensory system phosphorelay to upregulate 3', 5'-cyclic monophosphate (cAMP) production and transcription of virulence-associated genes. To dissect the specific interactions mediating the mechanochemical relay, we used affinity purification/mass spectrometry, directed co-immunoprecipitations in P. aeruginosa, single cell analysis of contact-dependent transcriptional reporters, subcellular localization and bacterial two hybrid assays. We demonstrate that FimL, a Chp chemosensory system accessory protein of unknown function, directly links the integral component of the TFP structural complex FimV, a peptidoglycan binding protein, with one of the Chp system output response regulators PilG. FimL and PilG colocalize at cell poles in a FimV-dependent manner. While PilG phosphorylation is required for TFP function and mechanochemical signaling, it is not required for polar localization or binding to FimL. Phylogenetic analysis reveals other bacterial species simultaneously encode TFP, the Chp system, FimL, FimV and adenylate cyclase homologs, suggesting that surface sensing may be widespread among TFP-expressing bacteria. We propose that FimL acts as a scaffold enabling spatial colocalization of TFP and Chp system components to coordinate signaling leading to cAMP-dependent upregulation of virulence genes on surface contact.
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