ArticleJournal of separation science2016
Quantitative analysis of N-acylphosphatidylethanolamine molecular species in rat brain using solid-phase extraction combined with reversed-phase chromatography and tandem mass spectrometry.
Article in Journal of separation science, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 8 papers.
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Who cites it
8 citing papers in PubMed.
- Chemical Composition and Biological Activity of Phenolic Compounds and Carotenoids Extracted from Yellow-Purple Polignano Carrots.Foods (Basel, Switzerland) · 2026Article
- Mass Spectrometry Detects Sphingolipid Metabolites for Discovery of New Strategy for Cancer Therapy from the Aspect of Programmed Cell Death.Metabolites · 2023Review
- Recommendations for good practice in MS-based lipidomics.Journal of lipid research · 2021Review
- Biosynthesis ofInternational journal of molecular sciences · 2020Article
- Lipidomics from sample preparation to data analysis: a primer.Analytical and bioanalytical chemistry · 2020Review
- Analysis of Phospholipids, Lysophospholipids, and Their Linked Fatty Acyl Chains in Yellow Lupin Seeds (Molecules (Basel, Switzerland) · 2020Article
- Lipidomics: Prospects from a technological perspective.Biochimica et biophysica acta. Molecular and cell biology of lipids · 2017Review
- Lipidomics by ultrahigh performance liquid chromatography-high resolution mass spectrometry and its application to complex biological samples.Journal of chromatography. B, Analytical technologies in the biomedical and life sciences · 2017Article
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Authors and funding
5 authors.
Funding
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Abstract
A novel method for the sensitive and selective identification and quantification of N-acylphosphatidylethanolamine molecular species was developed. Samples were prepared using a combination of liquid-liquid and solid-phase extraction, and intact N-acylphosphatidylethanolamine species were determined by reversed-phase high-performance liquid chromatography coupled to positive electrospray tandem mass spectrometry. As a result of their biological functions as precursors for N-acylethanolamines and as signaling molecules, tissue concentrations of N-acylphosphatidylethanolamines are very low, and their analysis is additionally hindered by the vast excess of other sample components. Our sample preparation methods are able to selectively separate the analytes of interest from any expected biological interferences. Finally, the highest selectivity is achieved by coupling chromatographic separation and two N-acyl chain specific selected reaction monitoring scans per analyte, enabling identification of both the N-acyl chain and the phosphatidylethanolamine moiety. The validated method is suitable for the reliable quantification of N-acylphosphatidylethanolamine species from rat brain with a lower limit of quantification of 10 pmol/g and a linear range up to 2300 pmol/g. In total, 41 N-acylphosphatidylethanolamine molecular species with six different N-acyl chains, amounting to a total concentration of 3 nmol/g, were quantified.
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