ArticleBacteriophage
T7 ejectosome assembly: A story unfolds.
Article in Bacteriophage. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
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Who cites it
19 citing papers in PubMed, 30 citations in OpenAlex.
- Targeting multidrug-resistant bacteria with genetic-information-free protein-only phages.bioRxiv : the preprint server for biology · 2025Article
- Deciphering shared receptor usage in genomically unrelated bacteriophages infecting hypervirulentFEMS microbes · 2025Article
- Atlas of Interactions Between Decoration Proteins and Major Capsid Proteins of Coliphage N4.Viruses · 2024Article
- Ejectosome ofPNAS nexus · 2024Article
- Revolving hexameric ATPases as asymmetric motors to translocate double-stranded DNA genome along one strand.iScience · 2023Review
- Abundant and cosmopolitan lineage of cyanopodoviruses lacking a DNA polymerase gene.The ISME journal · 2023Article
- Genomic Characterization of Twelve Lytic Bacteriophages Infecting Midgut Bacteria of Aedes aegypti.Current microbiology · 2022Article
- Evaluating the Phenotypic and Genomic Characterization of Some Egyptian Phages Infecting Shiga Toxin-ProducingBiology · 2022Article
- Viral Ejection Proteins: Mosaically Conserved, Conformational Gymnasts.Microorganisms · 2022Review
- Mechanisms and clinical importance of bacteriophage resistance.FEMS microbiology reviews · 2022Review
- Genomic characterization of four novel bacteriophages infecting the clinical pathogen Klebsiella pneumoniae.DNA research : an international journal for rapid publication of reports on genes and genomes · 2021Article
- Assisted assembly of bacteriophage T7 core components for genome translocation across the bacterial envelope.Proceedings of the National Academy of Sciences of the United States of America · 2021Article
- Cryo-EM structure of the periplasmic tunnel of T7 DNA-ejectosome at 2.7 Å resolution.Molecular cell · 2021Article
- Intravirion DNA Can Access the Space Occupied by the Bacteriophage P22 Ejection Proteins.Viruses · 2021Article
- A Biological Inventory of Prophages inFrontiers in microbiology · 2020Article
- States of phage T3/T7 capsids: buoyant density centrifugation and cryo-EM.Biophysical reviews · 2018Review
- Article
- Phage-Enabled Nanomedicine: From Probes to Therapeutics in Precision Medicine.Angewandte Chemie (International ed. in English) · 2017Review
- Article
Corrections and comments
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
T7 phage DNA is transported from the capsid into the host cytoplasm across the cell wall by an ejectosome comprised of the viral proteins gp14, gp15 and gp16. Prior to infection, these proteins form the so-called internal core in the mature virion. Gp16 was shown to associate with pure phospholipid bilayers while gp15 bound to DNA. A complex of both proteins appears as spiral-like rods in electron micrographs. It was also shown that the proteins gp15 and gp16 have the propensity to regain their full structure after thermal unfolding. From these observations it was concluded that (partial) unfolding of the proteins occurs during the translocation through the narrow portal of the phage capsid. After leaving the phage head, the proteins refold to form the ejectosome channel across the periplasm of the host. In this work, we analyzed the structure of gp15 and gp16 in presence of lipids and their stability toward chemical denaturants. A model to explain how the ejectosome might assemble in the host cell is discussed.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.