ArticleThe Journal of biological chemistry2016
Substrate Specificity of Purified Recombinant Chicken β-Carotene 9',10'-Oxygenase (BCO2).
Article in The Journal of biological chemistry, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 52 papers.
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Who cites it
52 citing papers in PubMed, 81 citations in OpenAlex.
- Limited appearance of apocarotenoids is observed in plasma after consumption of tomato juices: a randomized human clinical trial.The American journal of clinical nutrition · 2018Trial
- Dietary marigold-derived lutein produces dose- and time-dependent egg yolk enrichment and coordinated hepatic-ovarian responses in laying hens.Poultry science · 2026Article
- Not All Roads Lead to Red: The Genetics of Sexual Dichromatism in the Northern Cardinal (Cardinals cardinalis).Genome biology and evolution · 2026Article
- Review
- A regulatory 24-bp insertion at the BCO2 gene region is associated with yellow skin in chickens.Genetics, selection, evolution : GSE · 2026Article
- Bioconversion of carotenoids into high-value crocins using a marine sponge carotenoid cleavage dioxygenase.The New phytologist · 2026Article
- Transcriptome Analysis of Differentially Expressed Genes in Freshwater Pearl Mussel (Animals : an open access journal from MDPI · 2026Article
- A colorful legacy of hybridization in wood-warblers includes frequent sharing of carotenoid genes among species and genera.PLoS biology · 2025Article
- Absorption characteristics andRSC advances · 2025Article
- Unveiling BCO2 function in macular pigment metabolism: Mitochondrial processing and expression in the primate retina.Biochimica et biophysica acta. Molecular and cell biology of lipids · 2025Article
- Sequential introgression of a carotenoid processing gene underlies sexual ornament diversity in a genus of manakins.Science advances · 2024Article
- Imaging macular carotenoids and their related proteins in the human retina with confocal resonance Raman and fluorescence microscopy.Experimental eye research · 2024Article
- Tryptophan Promotes the Production of Xanthophyll Compounds in Yellow Abdominal Fat throughAnimals : an open access journal from MDPI · 2024Article
- Preterm Pigs Fed Donor Human Milk Have Greater Liver β-Carotene Concentrations than Pigs Fed Infant Formula.The Journal of nutrition · 2023Article
- Carotenoid enrichment in eggs: From biochemistry perspective.Animal nutrition (Zhongguo xu mu shou yi xue hui) · 2023Review
- Lycopene Accumulation in Transgenic Mice Lacking One or Both Carotenoid Cleaving Enzymes.The Journal of nutrition · 2023Article
- Mechanism for the selective uptake of macular carotenoids mediated by the HDL cholesterol receptor SR-BI.Experimental eye research · 2023Article
- Carotenoids in Drug Discovery and Medicine: Pathways and Molecular Targets Implicated in Human Diseases.Molecules (Basel, Switzerland) · 2022Review
- Molecular Properties of β-Carotene Oxygenases and Their Potential in Industrial Production of Vitamin A and Its Derivatives.Antioxidants (Basel, Switzerland) · 2022Review
- Tomatoes, Lycopene, and Prostate Cancer: What Have We Learned from Experimental Models?The Journal of nutrition · 2022Review
Corrections and comments
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Authors and funding
8 authors at 1 institution in 1 country.
Funding
Abstract
Provitamin A carotenoids are oxidatively cleaved by β-carotene 15,15'-dioxygenase (BCO1) at the central 15-15' double bond to form retinal (vitamin A aldehyde). Another carotenoid oxygenase, β-carotene 9',10'-oxygenase (BCO2) catalyzes the oxidative cleavage of carotenoids at the 9'-10' bond to yield an ionone and an apo-10'-carotenoid. Previously published substrate specificity studies of BCO2 were conducted using crude lysates from bacteria or insect cells expressing recombinant BCO2. Our attempts to obtain active recombinant human BCO2 expressed in Escherichia coli were unsuccessful. We have expressed recombinant chicken BCO2 in the strain E. coli BL21-Gold (DE3) and purified the enzyme by cobalt ion affinity chromatography. Like BCO1, purified recombinant chicken BCO2 catalyzes the oxidative cleavage of the provitamin A carotenoids β-carotene, α-carotene, and β-cryptoxanthin. Its catalytic activity with β-carotene as substrate is at least 10-fold lower than that of BCO1. In further contrast to BCO1, purified recombinant chicken BCO2 also catalyzes the oxidative cleavage of 9-cis-β-carotene and the non-provitamin A carotenoids zeaxanthin and lutein, and is inactive with all-trans-lycopene and β-apocarotenoids. Apo-10'-carotenoids were detected as enzymatic products by HPLC, and the identities were confirmed by LC-MS. Small amounts of 3-hydroxy-β-apo-8'-carotenal were also consistently detected in BCO2-β-cryptoxanthin reaction mixtures. With the exception of this activity with β-cryptoxanthin, BCO2 cleaves specifically at the 9'-10' bond to produce apo-10'-carotenoids. BCO2 has been shown to function in preventing the excessive accumulation of carotenoids, and its broad substrate specificity is consistent with this.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.