ArticleScientific reports2016
A pseudouridylation switch in rRNA is implicated in ribosome function during the life cycle of Trypanosoma brucei.
Article in Scientific reports, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 32 papers.
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Who cites it
32 citing papers in PubMed, 48 citations in OpenAlex.
- A small nucleolar RNA dictates the structure and function of translating ribosomes in Leishmania.Nature communications · 2026Article
- Proteomic Identification of Small-Subunit Ribosome Assembly Factors inJournal of proteome research · 2026Article
- Translational control of Toxoplasma gondii differentiation.Bioscience reports · 2026Review
- Seven wonders of RNA modification biology.RNA (New York, N.Y.) · 2026Review
- Impacts of ribosomal RNA sequence variation on gene expression and phenotype.Philosophical transactions of the Royal Society of London. Series B, Biological sciences · 2025Review
- The zebrafish (NAR genomics and bioinformatics · 2025Article
- The extensive mNature communications · 2024Article
- Structural and mechanistic insights into the function of Leishmania ribosome lacking a single pseudouridine modification.Cell reports · 2024Article
- A role for a Trypanosoma brucei cytosine RNA methyltransferase homolog in ribosomal RNA processing.PloS one · 2024Article
- A single pseudouridine on rRNA regulates ribosome structure and function in the mammalian parasite Trypanosoma brucei.Nature communications · 2023Article
- Multiple domains of the integral KREPA3 protein are critical for the structure and precise functions of RNA editing catalytic complexes inRNA (New York, N.Y.) · 2023Article
- mt-LAF3 is a pseudouridine synthase ortholog required for mitochondrial rRNA and mRNA gene expression in Trypanosoma brucei.International journal for parasitology · 2023Article
- Ribosome Specialization in Protozoa Parasites.International journal of molecular sciences · 2023Review
- Omics data integration facilitates target selection for new antiparasitic drugs against TriTryp infections.Frontiers in pharmacology · 2023Article
- Lytic Reactivation of the Kaposi's Sarcoma-Associated Herpesvirus (KSHV) Is Accompanied by Major Nucleolar Alterations.Viruses · 2022Article
- The plant epitranscriptome: revisiting pseudouridine and 2'-O-methyl RNA modifications.Plant biotechnology journal · 2022Review
- A long noncoding RNA promotes parasite differentiation in African trypanosomes.Science advances · 2022Article
- Experimental evolution links post-transcriptional regulation to Leishmania fitness gain.PLoS pathogens · 2022Article
- Spaceflight-Associated Changes of snoRNAs in Peripheral Blood Mononuclear Cells and Plasma Exosomes-A Pilot Study.Frontiers in cardiovascular medicine · 2022Article
- Genome instability drives epistatic adaptation in the human pathogenProceedings of the National Academy of Sciences of the United States of America · 2021Article
Corrections and comments
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Authors and funding
11 authors at 4 institutions in 3 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The protozoan parasite Trypanosoma brucei, which causes devastating diseases in humans and animals in sub-Saharan Africa, undergoes a complex life cycle between the mammalian host and the blood-feeding tsetse fly vector. However, little is known about how the parasite performs most molecular functions in such different environments. Here, we provide evidence for the intriguing possibility that pseudouridylation of rRNA plays an important role in the capacity of the parasite to transit between the insect midgut and the mammalian bloodstream. Briefly, we mapped pseudouridines (Ψ) on rRNA by Ψ-seq in procyclic form (PCF) and bloodstream form (BSF) trypanosomes. We detected 68 Ψs on rRNA, which are guided by H/ACA small nucleolar RNAs (snoRNA). The small RNome of both life cycle stages was determined by HiSeq and 83 H/ACAs were identified. We observed an elevation of 21 Ψs modifications in BSF as a result of increased levels of the guiding snoRNAs. Overexpression of snoRNAs guiding modification on H69 provided a slight growth advantage to PCF parasites at 30 °C. Interestingly, these modifications are predicted to significantly alter the secondary structure of the large subunit (LSU) rRNA suggesting that hypermodified positions may contribute to the adaption of ribosome function during cycling between the two hosts.
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