ArticleNature communications2016
Analytic framework for peptidomics applied to large-scale neuropeptide identification.
Article in Nature communications, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 69 papers.
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Who cites it
69 citing papers in PubMed.
- Small-protein Enrichment Assay Enables the Rapid, Unbiased Analysis of Over 100 Low Abundance Factors from Human Plasma.Molecular & cellular proteomics : MCP · 2019Trial
- Discriminating single-amino acid differences and post-translational modifications in negatively charged peptides with α-hemolysin nanopores.Journal of nanobiotechnology · 2026Article
- Mass Spectrometry-Based Peptidomics for the Discovery and Profiling of Endogenous Peptides in Crustacean Hemolymph.ACS omega · 2026Article
- Clinical peptidomics for respiratory diseases: matrices, workflows, and translation towards treatable traits, with a focus on COPD.Clinical proteomics · 2026Review
- Single cell transcriptomics of human weight loss links adipocyte NPY1R to control of lipolysis.Molecular metabolism · 2026Article
- NeuroPpred-MSN: A Neuropeptide Prediction Model Based on Multi-feature Fusion and Siamese Networks.Interdisciplinary sciences, computational life sciences · 2026Article
- Degradation graphs reveal hidden proteolytic activity in peptidomes.PLoS computational biology · 2026Article
- Identification and Characterization of Glycine- and Proline-Rich Antioxidant Peptides From Antler Residues Based on Peptidomics, Machine Learning, and Molecular Docking.Food science & nutrition · 2025Article
- Toward high-efficiency, low-resource, and explainable neuropeptide prediction with MSKDNP.Briefings in bioinformatics · 2025Article
- Discovery of peptides as key regulators of metabolic and cardiovascular crosstalk.Cell reports · 2025Review
- Research progress of peptides discovery and function in resistance to abiotic stress in plant.Stress biology · 2025Review
- Decoding Neuropeptide Complexity: Advancing Neurobiological Insights from Invertebrates to Vertebrates through Evolutionary Perspectives.ACS chemical neuroscience · 2025Review
- Quantitative Analysis of Neuropeptide Y (NPY) and C-Terminal Glycine-Extended NPY by Mass Spectrometry and Their Localization in the Developing and Sexual Adult Mouse Brains.ACS chemical neuroscience · 2025Article
- Cocaine-Induced Remodeling of the Rat Brain Peptidome: Quantitative Mass Spectrometry Reveals Anatomically Specific Patterns of Cocaine-Regulated Peptide Changes.ACS chemical neuroscience · 2025Article
- Unveiling Endogenous Serum Peptides as Potential Biomarkers for Hepatocellular Carcinoma in Patients with Liver Cirrhosis.Journal of proteome research · 2024Article
- Peptide clustering enhances large-scale analyses and reveals proteolytic signatures in mass spectrometry data.Nature communications · 2024Article
- Peptidomic analysis reveals novel peptide PDLC promotes cell proliferation in hepatocellular carcinoma via Ras/Raf/MEK/ERK pathway.Scientific reports · 2024Article
- iNP_ESM: Neuropeptide Identification Based on Evolutionary Scale Modeling and Unified Representation Embedding Features.International journal of molecular sciences · 2024Article
- Neuropeptidomics of the American LobsterJournal of proteome research · 2024Article
- An Updated Guide to the Identification, Quantitation, and Imaging of the Crustacean Neuropeptidome.Methods in molecular biology (Clifton, N.J.) · 2024Article
9 more citing papers are in PubMed but not listed here.
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Authors and funding
7 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Large-scale mass spectrometry-based peptidomics for drug discovery is relatively unexplored because of challenges in peptide degradation and identification following tissue extraction. Here we present a streamlined analytical pipeline for large-scale peptidomics. We developed an optimized sample preparation protocol to achieve fast, reproducible and effective extraction of endogenous peptides from sub-dissected organs such as the brain, while diminishing unspecific protease activity. Each peptidome sample was analysed by high-resolution tandem mass spectrometry and the resulting data set was integrated with publically available databases. We developed and applied an algorithm that reduces the peptide complexity for identification of biologically relevant peptides. The developed pipeline was applied to rat hypothalamus and identifies thousands of neuropeptides and their post-translational modifications, which is combined in a resource format for visualization, qualitative and quantitative analyses.
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